IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of IL-7 R alpha in human PMBCs using anti-IL-7 R alpha antibody. Image from verified customer review. Image using the Alexa Fluor 405 format of this antibody.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 22F3D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free Summary

• Immunogen: Amino acids 141-237 of human CD127 were used as the immunogen for the antibody.
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL7R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100. Use reported by customer review
  • Western Blot 2-5 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. The amino acid sequence used as immunogen for the antibody is present in two isoforms of CD127: 459 amino acids and 299 amino acids. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

• CD127 antigen
• CD127
• CD127ILRA
• CDw127
• IL-7 R alpha
• IL-7 receptor subunit alpha
• IL7R alpha
• IL-7R alpha
• IL-7R subunit alpha
• IL7R
• IL7RA
• IL-7Ra
• IL-7R-alpha
• interleukin 7 receptor alpha chain
• interleukin 7 receptor isoform H5-6
• interleukin 7 receptor
• interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7R alpha/CD127 Antibody (NBP2-22376)(1)

Publication using NBP2-22376

• Kolodziej M, Strauss S, Lazaridis A et al. Influence of glucose and insulin in human adipogenic differentiation models with adipose-derived stem cells Adipocyte 2019-12-01 [PMID: 31280651] (FLOW, Human)

Bioinformatics

• Gene Symbol: IL7R
• Uniprot:
  • NP_002176.2(Human)