IL-6R alpha Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-6R alpha Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
  Leu20-Asp358
  Accession # P08887
• Specificity: Detects human IL-6R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) IL‑6, recombinant mouse IL-6, recombinant porcine IL-6, and rhgp130 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL6R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human IL-6 R alpha Antibody (Catalog # MAB227)
  ELISA Detection:Human IL-6 R alpha Biotinylated Antibody (Catalog # BAF227)
  Standard: Recombinant Human IL-6 R alpha (Catalog # 227-SR)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody [Biotin]

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha . No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

1. Yamasaki et al. (1988) Science 241:825.
2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
3. Hibi et al. (1990) Cell 63:1149.
4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
5. Novick et al. (1989) J. Exp. Med. 170:1409.
6. Yasukawa et al. (1990) J. Biochem. 108:673.
7. Saito et al. (1991) J. Immunology 147:168.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6R alpha Antibody (BAF227)(10)

Publications using BAF227

• CM Flynn, B Kespohl, T Daunke, Y Garbers, S Düsterhöft, S Rose-John, J Haybaeck, J Lokau, S Aparicio-S, C Garbers Interleukin-6 controls recycling and degradation, but not internalization of its receptors The Journal of Biological Chemistry, 2021-02-19;0(0):100434. 2021-02-19 [PMID: 33610555] (ICC, Western Blot, Human)
• F Mimoto, K Tatsumi, S Shimizu, S Kadono, K Haraya, M Nagayasu, Y Suzuki, E Fujii, M Kamimura, A Hayasaka, H Kawauchi, K Ohara, M Matsushita, T Baba, H Susumu, T Sakashita, T Muraoka, K Aso, H Katada, E Tanaka, K Nakagawa, M Hasegawa, M Ayabe, T Yamamoto, S Tanba, T Ishiguro, T Kamikawa, T Nambu, T Kibayashi, Y Azuma, Y Tomii, A Kato, K Ozeki, N Murao, M Endo, J Kikuta, M Kamata-Sak, M Ishii, K Hattori, T Igawa Exploitation of Elevated Extracellular ATP to Specifically Direct Antibody to Tumor Microenvironment Cell Reports, 2020-12-22;33(12):108542. 2020-12-22 [PMID: 33357423] (ELISA Capture, Mouse)
• S Riethmuell, P Somasundar, JC Ehlers, CW Hung, CM Flynn, J Lokau, M Agthe, S Dsterh”ft, Y Zhu, J Gr”tzinger, I Lorenzen, T Koudelka, K Yamamoto, U Pickhinke, R Wichert, C Becker-Pau, M R„disch, A Albrecht, M Hessefort, D Stahnke, C Unverzagt, S Rose-John, A Tholey, C Garbers Proteolytic Origin of the Soluble Human IL-6R In Vivo and a Decisive Role of N-Glycosylation PLoS Biol, 2017-01-06;15(1):e2000080. 1/6/2017 [PMID: 28060820]
• Iwayanagi Y, Igawa T, Maeda A, Haraya K, Wada N, Shibahara N, Ohmine K, Nambu T, Nakamura G, Mimoto F, Katada H, Ito S, Tachibana T, Jishage K, Hattori K Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering. J Immunol, 2015-08-28;195(7):3198-205. 2015-08-28 [PMID: 26320252] (ELISA Development, Human)
• Komori M, Blake A, Greenwood M et al. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis. Ann Neurol 7/1/2015 [PMID: 25808056]
• Dusterhoft S, Hobel K, Oldefest M, Lokau J, Waetzig G, Chalaris A, Garbers C, Scheller J, Rose-John S, Lorenzen I, Grotzinger J A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor. J Biol Chem, 2014-04-30;289(23):16336-48. 2014-04-30 [PMID: 24790088] (ELISA Development (Detection), Human)
• Wood MW, Breitschwerdt EB, Gookin JL Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa. Infect. Immun., 2010-11-29;79(2):708-15. 2010-11-29 [PMID: 21115724] (IHC-Fr, Canine)
• Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5. 2004-12-01 [PMID: 15585763] (ELISA Development, Human)
• Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 2002-11-01;169(9):5244-51. 2002-11-01 [PMID: 12391243] (ELISA Development, Human)
• Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity, 2001-06-01;14(6):705-14. 2001-06-01 [PMID: 11420041] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL6R
• Uniprot:
  • P08887()