227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_lightbox"> 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_thumb" />

227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND₅₀ is typically 0.2-1 µg/mL." class="big_lightbox"> 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND₅₀ is typically 0.2-1 µg/mL." class="big_thumb" />

1830-SR) was serially diluted and captured by Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1830-SR) was serially diluted and captured by Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1830-SR) was serially diluted and captured by Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1830-SR) was serially diluted and captured by Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 5 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA). The ND₅₀ is typically 0.2-1.6 µg/mL." class="big_lightbox"> 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA). The ND₅₀ is typically 0.2-1.6 µg/mL." class="big_thumb" />

227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-6R alpha was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-6R alpha Monoclonal Antibody (Catalog # MAB18301) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgGF(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgGF(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="U937 human histiocytic lymphoma cell line was stained with Human IL-6 R alpha Monoclonal Antibody (Catalog # MAB227R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgGF(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Ra Monoclonal Antibody (Catalog # MAB227R). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_lightbox"> 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Ra Monoclonal Antibody (Catalog # MAB227R). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_thumb" />

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Polyclonal Antibody (Catalog # AB-227-NA). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Polyclonal Antibody (Catalog # AB-227-NA). The ND₅₀ is typically 1-4 µg/mL." alt="Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Polyclonal Antibody (Catalog # AB-227-NA). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

F0135) or unstained cells (open blue histogram). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0135) or unstained cells (open blue histogram). View our protocol for Staining Membrane-associated Proteins. " alt="HEK293 cells transfected with hIL-6Ra and eGFP were stained with Human Anti-Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Monoclonal Antibody (Catalog # MAB11482, filled histogram) followed by Allophycocyanin-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) or unstained cells (open blue histogram). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 Human cell line transfected with (A) Human IL-6R alpha or (B) irrelevant protein, and eGFP was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (100 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human IL-6 Ra (Research Grade Sarilumab) Monoclonal Antibody (Catalog # MAB10346). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_lightbox"> 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (100 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human IL-6 Ra (Research Grade Sarilumab) Monoclonal Antibody (Catalog # MAB10346). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-6R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB2271) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />