IL-6R alpha Products

227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox" target="_blank">

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_lightbox" target="_blank">

227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND₅₀ is typically 0.2-1 µg/mL." class="big_lightbox" target="_blank">

1830-SR) was serially diluted and captured by Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

1830-SR) was serially diluted and captured by Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">

227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA). The ND₅₀ is typically 0.2-1.6 µg/mL." class="big_lightbox" target="_blank">

227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

406-ML) is immobilized at 2.00 μg/mL (100 μL/well). Catalog # 1830-SR Recombinant Mouse IL-6R alpha (aa 20-357) Protein binds with (306-ML) at an ED50 of 30.0-300 ng/mL." class="big_lightbox" target="_blank">

7270-IL) binds with an ED₅₀ of 7.5-45 ng/mL." class="big_lightbox" target="_blank">