IL-6R alpha Antibody [Unconjugated]

Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND50 is typically 0.2-1 µg/mL.

IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-6R alpha Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha
  Leu20-Glu357
  Accession # P22272
• Specificity: Detects mouse IL-6R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑6, recombinant human IL-6 R and rmgp130 is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il6ra
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.2-1 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse IL-6 R alpha Antibody (Catalog # AF1830)
  ELISA Detection: Mouse IL-6 R alpha Biotinylated Antibody (Catalog # BAF1830)
  Standard: Recombinant Mouse IL-6 R alpha (Catalog # 1830-SR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody [Unconjugated]

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

1. Yamasaki, K. et al. (1988) Science 241:825.
2. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
3. Hibi, M. et al. (1990) Cell 63:1149.
4. Saito, M. et al. (1992) J. Immunol. 148:4066.
5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
6. Jones, S.A. et al. (2001) FASEB J. 15:43.
7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6R alpha Antibody (AF1830)(12)

Publications using AF1830

• D Zhao, L Cai, X Lu, X Liang, J Li, P Chen, M Ittmann, X Shang, S Jiang, H Li, C Meng, I Flores, JH Song, JW Horner, Z Lan, CJ Wu, J Li, Q Chang, KC Chen, G Wang, P Deng, DJ Spring, YA Wang, RA DePinho Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer Cancer Discov, 2020;0(0):. 2020 [PMID: 32385075] (IHC, Human)
• H Huang, Y Zhang, V Gallegos, N Sorrelle, MM Zaid, J Toombs, W Du, S Wright, M Hagopian, Z Wang, AN Hosein, AA Sathe, C Xing, EJ Koay, KE Driscoll, RA Brekken Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer EMBO Mol Med, 2019;0(0):e10515. 2019 [PMID: 31609088] (Western Blot, Human)
• AJ St Leger, AM Hansen, H Karauzum, R Horai, CR Yu, A Laurence, KD Mayer-Barb, P Silver, R Villasmil, C Egwuagu, SK Datta, RR Caspi STAT-3-independent production of IL-17 by mouse innate-like ?? T cells controls ocular infection J. Exp. Med., 2018;0(0):. 2018 [PMID: 29490936] (Neutralization, Mouse)
• X Wang, EB Miller, M Goswami, P Zhang, KE Ronning, SJ Karlen, RJ Zawadzki, EN Pugh, ME Burns Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130 J Neuroinflammation, 2017;14(1):121. 2017 [PMID: 28645275] (Neutralization, Mouse)
• P Arnold, I Boll, M Rothaug, N Schumacher, F Schmidt, R Wichert, J Schneppenh, J Lokau, U Pickhinke, T Koudelka, A Tholey, B Rabe, J Scheller, R Lucius, C Garbers, S Rose-John, C Becker-Pau Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling Sci Rep, 2017;7(0):44053. 2017 [PMID: 28276471]
• Nana Owusu-Boai Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner Immun Inflamm Dis, 2016;4(4):413-426. 2016 [PMID: 27980776] (Bioassay, Mouse)
• Garbers C, Janner N, Chalaris A, Moss ML, Floss DM, Meyer D, Koch-Nolte F, Rose-John S, Scheller J Species Specificity of ADAM10 and ADAM17 Proteins in Interleukin-6 (IL-6) Trans-signaling and Novel Role of ADAM10 in Inducible IL-6 Receptor Shedding. J. Biol. Chem., 2011;286(17):14804-11. 2011 [PMID: 21454673] (Western Blot, Mouse)
• Walker F, Zhang HH, Matthews V, Weinstock J, Nice EC, Ernst M, Rose-John S, Burgess AW IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice. Blood, 2007;111(8):3978-85. 2007 [PMID: 18156493] (Western Blot, Mouse)
• Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J Transgenic blockade of interleukin 6 transsignaling abrogates inflammation. Blood, 2007;111(3):1021-8. 2007 [PMID: 17989316] (ELISA Development, Mouse)
• Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Blood, 2007;110(6):1748-55. 2007 [PMID: 17567983] (ELISA Development, Mouse)
• Kurooka M, Kaneda Y Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res., 2007;67(1):227-36. 2007 [PMID: 17210703] (Neutralization, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005;302(1):172-81. 2005 [PMID: 16023134] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Il6ra
• Uniprot:
  • P22272()