IL-6R alpha Antibody [Unconjugated]


Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 more
IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained more
IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)

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IL-6R alpha Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha
Accession # P22272
Detects mouse IL-6R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑6, recombinant human IL-6 R and rmgp130 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.2-1 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse IL-6 R alpha Antibody (Catalog # AF1830)
ELISA Detection: Mouse IL-6 R alpha Biotinylated Antibody (Catalog # BAF1830)
Standard: Recombinant Mouse IL-6 R alpha (Catalog # 1830-SR)
Read Publications using
AF1830 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody [Unconjugated]

  • CD126
  • gp80
  • IL-6 R alpha
  • IL6Q
  • IL6R alpha
  • IL-6R alpha
  • IL6R
  • IL-6R-1
  • IL6RA
  • IL-6Ra
  • IL6RQ
  • interleukin 6 receptor


Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

  1. Yamasaki, K. et al. (1988) Science 241:825.
  2. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
  3. Hibi, M. et al. (1990) Cell 63:1149.
  4. Saito, M. et al. (1992) J. Immunol. 148:4066.
  5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
  6. Jones, S.A. et al. (2001) FASEB J. 15:43.
  7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-6R alpha Antibody (AF1830)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Bioassay, ELISA Development, IHC, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF1830 Applications Species
D Zhao, L Cai, X Lu, X Liang, J Li, P Chen, M Ittmann, X Shang, S Jiang, H Li, C Meng, I Flores, JH Song, JW Horner, Z Lan, CJ Wu, J Li, Q Chang, KC Chen, G Wang, P Deng, DJ Spring, YA Wang, RA DePinho Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer Cancer Discov, 2020;0(0):. 2020 [PMID: 32385075] (IHC, Human) IHC Human
H Huang, Y Zhang, V Gallegos, N Sorrelle, MM Zaid, J Toombs, W Du, S Wright, M Hagopian, Z Wang, AN Hosein, AA Sathe, C Xing, EJ Koay, KE Driscoll, RA Brekken Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer EMBO Mol Med, 2019;0(0):e10515. 2019 [PMID: 31609088] (Western Blot, Human) Western Blot Human
AJ St Leger, AM Hansen, H Karauzum, R Horai, CR Yu, A Laurence, KD Mayer-Barb, P Silver, R Villasmil, C Egwuagu, SK Datta, RR Caspi STAT-3-independent production of IL-17 by mouse innate-like ?? T cells controls ocular infection J. Exp. Med., 2018;0(0):. 2018 [PMID: 29490936] (Neutralization, Mouse) Neutralization Mouse
X Wang, EB Miller, M Goswami, P Zhang, KE Ronning, SJ Karlen, RJ Zawadzki, EN Pugh, ME Burns Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130 J Neuroinflammation, 2017;14(1):121. 2017 [PMID: 28645275] (Neutralization, Mouse) Neutralization Mouse
P Arnold, I Boll, M Rothaug, N Schumacher, F Schmidt, R Wichert, J Schneppenh, J Lokau, U Pickhinke, T Koudelka, A Tholey, B Rabe, J Scheller, R Lucius, C Garbers, S Rose-John, C Becker-Pau Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling Sci Rep, 2017;7(0):44053. 2017 [PMID: 28276471]
Nana Owusu-Boai Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner Immun Inflamm Dis, 2016;4(4):413-426. 2016 [PMID: 27980776] (Bioassay, Mouse) Bioassay Mouse
Garbers C, Janner N, Chalaris A, Moss ML, Floss DM, Meyer D, Koch-Nolte F, Rose-John S, Scheller J Species Specificity of ADAM10 and ADAM17 Proteins in Interleukin-6 (IL-6) Trans-signaling and Novel Role of ADAM10 in Inducible IL-6 Receptor Shedding. J. Biol. Chem., 2011;286(17):14804-11. 2011 [PMID: 21454673] (Western Blot, Mouse) Western Blot Mouse
Walker F, Zhang HH, Matthews V, Weinstock J, Nice EC, Ernst M, Rose-John S, Burgess AW IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice. Blood, 2007;111(8):3978-85. 2007 [PMID: 18156493] (Western Blot, Mouse) Western Blot Mouse
Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J Transgenic blockade of interleukin 6 transsignaling abrogates inflammation. Blood, 2007;111(3):1021-8. 2007 [PMID: 17989316] (ELISA Development, Mouse) ELISA Development Mouse
Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Blood, 2007;110(6):1748-55. 2007 [PMID: 17567983] (ELISA Development, Mouse) ELISA Development Mouse
Show All 12 Publications.

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Biotin BAF1830

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Gene Symbol Il6ra