IL-6R alpha Antibody (255821)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
255821
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

IL-6R alpha Antibody (255821) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha
Leu20-Glu357
Accession # P22272
Specificity
Detects mouse IL‑6 R alpha in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human IL-6 R alpha is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Il6ra
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1830 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody (255821)

  • CD126
  • gp80
  • IL-6 R alpha
  • IL6Q
  • IL6R alpha
  • IL-6R alpha
  • IL6R
  • IL-6R-1
  • IL6RA
  • IL-6Ra
  • IL6RQ
  • interleukin 6 receptor

Background

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). Mature mouse IL-6 R alpha is a type I transmembrane protein of 460 amino acids (aa) that contains a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSxWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds  IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

  1. Yamasaki, K. et al. (1988) Science 241:825.
  2. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
  3. Hibi, M. et al. (1990) Cell 63:1149.
  4. Saito, M. et al. (1992) J. Immunol. 148:4066.
  5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
  6. Jones, S.A. et al. (2001) FASEB J. 15:43.
  7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-6R alpha Antibody (MAB1830)(3)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1830U
Alexa Fluor 405 FAB1830V
Alexa Fluor 488 FAB1830G
Alexa Fluor 594 FAB1830T
Alexa Fluor 647 FAB1830R
Alexa Fluor 700 FAB1830N
Alexa Fluor 750 FAB1830S
Biotin BAM1830

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Bioinformatics

Gene Symbol Il6ra
Uniprot