IL-6R alpha Antibody (17506) [Unconjugated]

Images

 
U937 human histiocytic lymphoma cell line was labeled with HumanIL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). ...read more
Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-HumanIL-6R alpha ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
Clone
17506
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

IL-6R alpha Antibody (17506) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Leu20-Asp339
Accession # P08887
Specificity
Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL6R
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.04-0.2 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human IL-6 Ralpha Biotinylated Antibody (Catalog number BAF227)
Standard: Recombinant Human IL-6 Ralpha (Catalog number 227-SR)
Reviewed Applications
Read 1 Review rated 5
using
MAB227 in the following applications:

Publications
Read Publications using
MAB227 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody (17506) [Unconjugated]

  • CD126
  • gp80
  • IL-6 R alpha
  • IL6Q
  • IL6R alpha
  • IL-6R alpha
  • IL6R
  • IL-6R-1
  • IL6RA
  • IL-6Ra
  • IL6RQ
  • interleukin 6 receptor

Background

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-6R alpha Antibody (MAB227)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, In Vivo, Neutralization, Western Blot.


Filter By Application
ELISA Development
(3)
Flow Cytometry
(3)
In Vivo
(1)
Neutralization
(8)
Western Blot
(3)
All Applications
Filter By Species
Human
(18)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB227 Applications Species
C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30040142] (Neutralization, Human) Neutralization Human
IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016;90(0):88-95. 2016 [PMID: 27863335] (Flow Cytometry, Human) Flow Cytometry Human
Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium PLoS ONE, 2016;11(5):e0155161. 2016 [PMID: 27171357] (Neutralization, Human) Neutralization Human
Sommer A, Fabri M Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE, 2015;10(6):e0130395. 2015 [PMID: 26107738] (Neutralization, Human) Neutralization Human
Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells. J Immunol, 2015;194(6):2578-86. 2015 [PMID: 25667417] (Neutralization, Human) Neutralization Human
Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor. Oncogene, 2014;33(9):1158-66. 2014 [PMID: 23455323] (Neutralization, Human) Neutralization Human
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Neutralization, Human) Neutralization Human
Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat. Med., 2011;17(11):1498-503. 2011 [PMID: 22037646] (In Vivo, Human) In Vivo Human
Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Nat. Immunol., 2009;10(8):857-63. 2009 [PMID: 19578369] (Neutralization, Human) Neutralization Human
Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res., 2009;69(1):329-37. 2009 [PMID: 19118018] (Flow Cytometry, Human) Flow Cytometry Human
Show All 18 Publications.

Review for IL-6R alpha Antibody (MAB227) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB227:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot IL-6R alpha MAB227
Enlarge
5
reviewed by:
Anonymous
WB Human 04/04/2020
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Summary

ApplicationWestern Blot
Sample TestedHuman liver cancer cell line (HuH7),MDA-MB-231
SpeciesHuman
LotIQ0916021

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Recent Reviews

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1
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Anonymous
04/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol IL6R
Uniprot