IL-6R alpha Antibody (17506) [Unconjugated]


U937 human histiocytic lymphoma cell line was labeled with HumanIL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by more
Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). more
Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-HumanIL-6R alpha more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ELISA(Cap)

IL-6R alpha Antibody (17506) [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Accession # P08887
Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
Details of Functionality
ActivityAssaywCitation not found None None None None
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.04-0.2 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human IL-6 Ralpha Biotinylated Antibody (Catalog number BAF227)
Standard: Recombinant Human IL-6 Ralpha (Catalog number 227-SR)
Reviewed Applications
Read 1 Review rated 5
MAB227 in the following applications:

Read Publications using
MAB227 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody (17506) [Unconjugated]

  • CD126
  • gp80
  • IL-6 R alpha
  • IL6Q
  • IL6R alpha
  • IL-6R alpha
  • IL6R
  • IL-6R-1
  • IL6RA
  • IL-6Ra
  • IL6RQ
  • interleukin 6 receptor


The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Po
Applications: Flow, ICC/IF, IF
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IP
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for IL-6R alpha Antibody (MAB227)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, In Vivo, Neutralization, Western Blot.

Filter By Application
ELISA Development
Flow Cytometry
In Vivo
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB227 Applications Species
C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30040142] (Neutralization, Human) Neutralization Human
IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016;90(0):88-95. 2016 [PMID: 27863335] (Flow Cytometry, Human) Flow Cytometry Human
Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium PLoS ONE, 2016;11(5):e0155161. 2016 [PMID: 27171357] (Neutralization, Human) Neutralization Human
Sommer A, Fabri M Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE, 2015;10(6):e0130395. 2015 [PMID: 26107738] (Neutralization, Human) Neutralization Human
Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells. J Immunol, 2015;194(6):2578-86. 2015 [PMID: 25667417] (Neutralization, Human) Neutralization Human
Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor. Oncogene, 2014;33(9):1158-66. 2014 [PMID: 23455323] (Neutralization, Human) Neutralization Human
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Neutralization, Human) Neutralization Human
Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat. Med., 2011;17(11):1498-503. 2011 [PMID: 22037646] (In Vivo, Human) In Vivo Human
Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Nat. Immunol., 2009;10(8):857-63. 2009 [PMID: 19578369] (Neutralization, Human) Neutralization Human
Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res., 2009;69(1):329-37. 2009 [PMID: 19118018] (Flow Cytometry, Human) Flow Cytometry Human
Show All 18 Publications.

Review for IL-6R alpha Antibody (MAB227) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB227:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot IL-6R alpha MAB227
reviewed by:
WB Human 04/04/2020


ApplicationWestern Blot
Sample TestedHuman liver cancer cell line (HuH7),MDA-MB-231

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-6R alpha Antibody (MAB227) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional IL-6R alpha Products

Bioinformatics Tool for IL-6R alpha Antibody (MAB227)

Discover related pathways, diseases and genes to IL-6R alpha Antibody (MAB227). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-6R alpha Antibody (MAB227)

Discover more about diseases related to IL-6R alpha Antibody (MAB227).

Pathways for IL-6R alpha Antibody (MAB227)

View related products by pathway.

PTMs for IL-6R alpha Antibody (MAB227)

Learn more about PTMs related to IL-6R alpha Antibody (MAB227).

Blogs on IL-6R alpha

There are no specific blogs for IL-6R alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Application: WB
Species: Human


Gene Symbol IL6R