IL-6R alpha Antibody (17506) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human IL-6R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB227P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P). View our protocol for Staining Membrane-associated Proteins.

U937 cells were stained with Mouse Anti-Human IL-6R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB227P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 17506
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-6R alpha Antibody (17506) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
  Leu20-Asp339
  Accession # P08887
• Specificity: Detects human IL-6R alpha in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha , recombinant mouse (rm) IL-1 alpha , rhIL-1 beta , rmIL-1 beta , rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody (17506) [Phycoerythrin]

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

The multi-functional factor Interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha . No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

1. Yamasaki, K. et al. (1988) Science 241:825.
2. Baumann, M. et al. (1990) J. Biol. Chem. 265:19853.
3. Hibi, M. et al. (1990) Cell 63:1149.
4. Schooltink, H. et al. (1991) Eur. J. Biochem. 277:659.
5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
6. Yasukawa, K. et al. (1990) J. Biochem. 108:673.
7. Saito, T. et al. (1991) J. Immunology 147:168.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6R alpha Antibody (FAB227P)(1)

Publication using FAB227P

• Morandi F, Airoldi I, Pistoia V IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-gamma release by autologous NK cells. J Immunol Res, 2014-03-10;2014(0):938561. 2014-03-10 [PMID: 24741633] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL6R
• Uniprot:
  • P08887()