Human IL-6 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-6 positive serum. There was no crossreactivity detected
|Standard Curve Range
1.56 - 100 pg/mL
The recovery ranged from 78% to 105% with an overall mean recovery of 88%. Please see protocol for more specific Spike Recovery details.
ELISA Kit (Colorimetric)
The human IL-6 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-6.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6
- Amplification Diluent Concentrate (2x)
- Amplification Reagent I*
- Amplification Reagent II
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- * reagent contains ethyl alcohol
- Control high, lyophilized
- Control low, lyophilized
- Biotin-Conjugate anti-human IL-6 monoclonal antibody
- Human IL-6 Standard lyophilized, 200 pg/mL upon reconstitution
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
Alternate Names for Human IL-6 ELISA Kit (Colorimetric)
- B cell stimulatory factor-2
- B-cell differentiation factor
- BSF2CTL differentiation factor
- HGFHSFIFNB2Hybridoma growth factor
- IL-6B-cell stimulatory factor 2
- Interferon beta-2
- interleukin 6 (interferon, beta 2)
- interleukin BSF-2
Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide (LPS) or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. IL-6 is involved in: - the induction of B-cell differentiation - the induction of acute phase proteins in liver cells - growth promotion of myeloma/plasmacytoma/hybridoma cells - induction of IL-2 and IL-2 receptor expression - proliferation and differentiation of T cells - inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages - enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor - induction of mesangial cell growth - induction of neural differentiation of PC 12 cells - induction of keratinocyte growth The abnormal production of IL-6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL-6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL-6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu, Rt
Applications: WB, IP, ICC, ICFlow, KO
Species: Hu, Mu, Rt
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
Publications for IL-6 ELISA Kit (NBP1-89869) (0)
There are no publications for IL-6 ELISA Kit (NBP1-89869).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for IL-6 ELISA Kit (NBP1-89869) (0)
There are no reviews for IL-6 ELISA Kit (NBP1-89869).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for IL-6 ELISA Kit (NBP1-89869). (Showing 1 - 1 of 1 FAQ).
I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
- We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
Additional IL-6 Products
Bioinformatics Tool for IL-6 ELISA Kit (NBP1-89869)
Discover related pathways, diseases and genes to IL-6 ELISA Kit (NBP1-89869). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on IL-6.
The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins. The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein... Read full blog post.
The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post.
VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g... Read full blog post.
TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss... Read full blog post.
MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b... Read full blog post.
"Get the skinny" on Adiponectin & Leptin Antibodies
We at Novus Biologicals have an extensive antibody catalog covering lipid and metabolism research, including Adiponectin and leptin antibody products.Excess body fat is linked to many human diseases, including IRS (insulin resistance syndrome), type... Read full blog post.