Human IL-6 ELISA Kit (Colorimetric)

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ELISA: IL-6 ELISA Kit [NBP1-89869] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
1.56 - 100 pg/mL
Sensitivity
0.92 pg/mL

Order Details

Human IL-6 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-6 positive serum. There was no crossreactivity detected
Standard Curve Range
1.56 - 100 pg/mL
Sensitivity
0.92 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.2%
Intra-Assay
3.4%
Spike Recovery
The recovery ranged from 78% to 105% with an overall mean recovery of 88%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL6

Applications/Dilutions

Application Notes
The human IL-6 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-6.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6
  2. Amplification Diluent Concentrate (2x)
  3. Amplification Reagent I*
  4. Amplification Reagent II
  5. Adhesive Films
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. * reagent contains ethyl alcohol
  8. Human IL-6 Standard lyophilized, 200 pg/mL upon reconstitution
  9. Control high, lyophilized
  10. Control low, lyophilized
  11. Biotin-Conjugate anti-human IL-6 monoclonal antibody
  12. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  13. Substrate Solution (tetramethyl-benzidine)
  14. Streptavidin-HRP
  15. Stop Solution (1M Phosphoric acid)
  16. Sample Diluent

Alternate Names for Human IL-6 ELISA Kit (Colorimetric)

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta-2
  • IL6
  • IL-6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide (LPS) or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. IL-6 is involved in: - the induction of B-cell differentiation - the induction of acute phase proteins in liver cells - growth promotion of myeloma/plasmacytoma/hybridoma cells - induction of IL-2 and IL-2 receptor expression - proliferation and differentiation of T cells - inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages - enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor - induction of mesangial cell growth - induction of neural differentiation of PC 12 cells - induction of keratinocyte growth The abnormal production of IL-6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL-6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL-6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 ELISA Kit (NBP1-89869). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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Bioinformatics

Gene Symbol IL6
Entrez