Recombinant Mouse IL-5 Protein

Product Discontinued

Recombinant Mouse IL-5 Protein Summary

• Description: Recombinant bioactive protein for Mouse IL-5
  
  

  Source:E. coli
  Amino Acid Sequence:(P04401)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: Mouse IL-5 is fully biologically active as compared to standard. The ED50, as calculated by the dose-dependent induction of TF-1 cell proliferation, is 1.7ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL5
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 26.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 20 mM Sodium phosphate buffer (pH 7.5).
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterile H2O to 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Alternate Names for Recombinant Mouse IL-5 Protein

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

Mouse Interleukin-5 (mIL-5) is a glycoprotein also known as EDF and TRF. It is involved in B-cell growth and eosinophil activation. IL-5 has been shown to be expressed in Th2, mast cells and eosinophils and is regulated by GATA-3, in addition to other transcription factors. IL-5 signals its biological effect through interaction with the IL-5 receptor (IL-5R). Recombinant mIL-5 is produced in E.Coli, comprised of two 113 amino acid chains forming a disulfide-linked homodimer with a molecular weight of 26.2 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL5