Non-human Primate IL-5 ELISA Kit

ELISA: Non-human Primate IL-5 ELISA kit [NBP3-11734] - Representative standard curve.

• Reactivity: Pm-NHP
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernatants
• Standard Curve Range: 3.16 - 1000 pg/mL
• Sensitivity: 1 pg/mL

Non-human Primate IL-5 ELISA Kit Summary

• Standard Curve Range: 3.16 - 1000 pg/mL
• Sensitivity: 1 pg/mL
• Inter-Assay: %CV < 13.4
• Intra-Assay: %CV < 3.6
• Spike Recovery: 69 - 130%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit
• Gene: IL5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Non-human Primate IL-5 ELISA Kit

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-5 ELISA Kit (NBP3-11734). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL5