Rat IL-5 ELISA Kit (Colorimetric)

ELISA: Rat IL-5 ELISA Kit (Colorimetric) [NBP3-06769] - Samples were spiked with high concentrations of Rat IL-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat IL-5 ELISA Kit (Colorimetric) [NBP3-06769] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat IL-5 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat IL-5 in samples. No significant cross-reactivity or interference between Rat IL-5 and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.15
• Intra-Assay: 5.27
• Spike Recovery: 85 - 107 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL5

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IL-5 ELISA Kit (Colorimetric)

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Review for IL-5 ELISA Kit (NBP3-06769) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 06/13/2022

Summary

• Sample Tested: Heparin Plasma,Serum
• Lot: B3UGWXZJG3

Comments

• Comments: ZDF rats, diabetic model. Influence of vitamin K2 on cytokine expression with regard to disease pathogenesis. Dilutions between 1:3 and 1:5 proved optimal.

FAQs for IL-5 ELISA Kit (NBP3-06769). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL5