Mouse IL-5 ELISA Kit (Colorimetric)

ELISA: IL-5 ELISA Kit [Biotin] [NBP1-92672] - Standard curve

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 3.3 pg/mL

Mouse IL-5 ELISA Kit (Colorimetric) Summary

• Description: The mouse IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-5.
• Specificity: The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-5 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 3.3 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-5 ELISA Kit (Colorimetric)

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

Mouse IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stew cells, induction of mIL-2R expression on both normal splenic and activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. mIL-5 has been shown to be produced exclusively by the TH2 subset of mouse TH clones. The spectrum of biological activities of mIL-5, on B cells as well as other cell types suggests that mIL-5 plays an important functional role in the activities of TH2 cells. mIL-5 is a heavily glycosylated glycoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL5