IL-5 Antibody Pair [HRP]

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Sandwich ELISA: IL-5 Antibody Pair [HRP] [NBP2-79517] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, S-ELISA
Clonality
Monoclonal
Host
Rabbit
Conjugate
HRP
Standard Curve Range
3.91-250 pg/ml
Sensitivity
3.91 pg/ml

Order Details

IL-5 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human IL-5.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IL-5.
Standard Curve Range
3.91-250 pg/ml
Sensitivity
3.91 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Rabbit
Gene
IL5

Applications/Dilutions

Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 250 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Standard: Each vial contains 45 ng of recombinant Human IL-5.
  2. Capture Antibody: 1 mg/ml of rabbit anti-Human IL-5 monoclonal antibody (in PBS, pH 7.4).
  3. Detection Antibody: 0.2 mg/ml of rabbit anti-Human IL-5 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).

Alternate Names for IL-5 Antibody Pair [HRP]

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Other Available Formats

HRP NBP2-79517

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Bioinformatics

Gene Symbol IL5