IL-5 Antibody (299257)

Recombinant Rat IL‑5 (Catalog # 545-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑5 Monoclonal Antibody (Catalog # MAB5451). The ND50 is typically 0.02‑0.08 µg/mL.

• Reactivity: Rt
• Applications: B/N
• Clone: 299257
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-5 Antibody (299257) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5
  Met20-Val132
  Accession # Q08125
• Specificity: Detects rat IL-5 in direct ELISAs. In direct ELISAs, 50% cross-reactivity with mouse IL-5 is observed and no cross‑reactivity with human, equine, canine, feline, or rhesus monkey IL-5 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.02-.0.08 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (299257)

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for rat IL-5 encodes a signal peptide and a 113 amino acid (aa) secreted mature protein. Mature rat IL-5 shares 70%, 94%, 56%, 63%, 59% and 59%, aa sequence identity with human, mouse, canine, equine, feline and porcine IL-5, respectively. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
11. Tavernier, J. et al. (1991) Cell 66:1175.
12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
14. Tavernier, J. et al. (2000) Blood 95:1600.
15. Kopf, M. et al. (1996) Immunity 4:15.
16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
17. Denburg, J. A. et al. (1991) Blood 77:1462.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL5
• Uniprot:
  • Q08125()