IL-4R alpha Antibody [Phycoerythrin]

Mouse splenocytes were stained with Goat Anti-Mouse IL-4 Ra PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB530P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Phycoerythrin

IL-4R alpha Antibody [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-4 R alpha
• Specificity: Detects mouse IL-4 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) IL-4 R, rhIL-9 R, rhIL-13 R alpha 1, and recombinant mouse IL-13 R alpha 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL4RA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4R alpha Antibody [Phycoerythrin]

• CD124 antigen
• CD124
• IL-4 R alpha
• IL-4 receptor subunit alpha
• IL4R alpha
• IL-4R alpha
• IL-4R subunit alpha
• IL4R
• IL-4Ra
• IL4RACD124
• IL-4R-alpha
• interleukin 4 receptor
• interleukin-4 receptor alpha chain
• interleukin-4 receptor subunit alpha

Background

Interleukin 4 is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils. The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors. As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component ( beta chain) of the IL-4 receptor complex.

cDNA clones for the ligand binding chain (IL-4 R) of both the mouse and human high affinity IL-4 receptors have been isolated. The human or mouse IL-4R is an approximately 140 kDa transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction. In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing and that encodes a soluble secreted form of IL-4 R has been isolated from mouse cells, but not yet from human sources. A naturally-occurring soluble form of the IL-4 R has also been identified in mouse biological fluids and murine cell culture supernatants.

Native or recombinant murine soluble IL-4 R, as well as recombinant human soluble IL-4 R, can bind IL-4 with the same affinity as the membrane bound IL-4 R. Soluble IL-4 R is a competitive inhibitor of IL-4 and has been shown to neutralize effectively many IL-4-mediated responses both in vivo and in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-4R alpha Antibody (FAB530P)(2)

Publications using FAB530P

• Q Zhang, W Zhu, F Xu, X Dai, L Shi, W Cai, H Mu, TK Hitchens, LM Foley, X Liu, F Yu, J Chen, Y Shi, RK Leak, Y Gao, J Chen, X Hu The interleukin-4/PPARγ signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury PLoS Biol., 2019-06-21;17(6):e3000330. 2019-06-21 [PMID: 31226122] (Flow Cytometry, Mouse)
• Natascha Köstlin-Gille, Stefanie Dietz, Julian Schwarz, Bärbel Spring, Jan Pauluschke-Fröhlich, Christian F. Poets et al. HIF-1 alpha -Deficiency in Myeloid Cells Leads to a Disturbed Accumulation of Myeloid Derived Suppressor Cells (MDSC) During Pregnancy and to an Increased Abortion Rate in Mice Frontiers in Immunology 2/5/2019 [PMID: 30804946]

Bioinformatics

• Gene Symbol: IL4RA