Non-human Primate IL-4 ELISA Kit Summary
| Standard Curve Range |
3.16 - 1000 pg/mL |
| Sensitivity |
3 pg/mL |
| Assay Type |
Sandwich-ELISA |
| Inter-Assay |
%CV < 18.1 |
| Intra-Assay |
%CV < 7.3 |
| Spike Recovery |
93 - 127% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit |
| Gene |
IL4 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive plate covers (3 EA)
- Biotinylated detection mAb (IL4-II)
- ELISA diluent
- Pre-coated ELISA strip plates (mAb IL4-I)
- Recombinant human IL-4 ELISA standard
- Standard reconstitution buffer
- Stop solution
- Streptavidin-HRP diluent
- Streptavidin-HRP
- Substrate (TMB)
- Wash buffer concentrate (20X)
|
Alternate Names for Non-human Primate IL-4 ELISA Kit
Background
IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-4 ELISA Kit (NBP3-11724). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.