IL-4 Antibody (855824)

Recombinant Rabbit IL‑4 (Catalog # 6939-RB) induces proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by IL‑4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit IL‑4 Monoclonal Antibody (Catalog # MAB6939). The ND50 is typically 0.25-1.25 μg/mL.

• Reactivity: Rb
• Applications: B/N
• Clone: 855824
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-4 Antibody (855824) Summary

• Immunogen: E. coli-derived recombinant rabbit IL-4
  Arg25-Ser147
  Accession # Q9MZR8
• Specificity: Detects rabbit IL-4 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4 Antibody (855824)

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rabbit IL-4 is synthesized with a 24 aa signal sequence (5). Mature rabbit IL-4 shares 47%, 56%, 39% and 40% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6‑8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
4. Redfield, C. et al. (1991) Biochemistry 30:11029.
5. Perkins, H.D. et al. (2000) Cytokine 12:555.
6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL4
• Uniprot:
  • Q9MZR8()