IL-4 Antibody (56567) [Alexa Fluor® 488]

• Reactivity: Rt
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 56567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

IL-4 Antibody (56567) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant rat IL‑4
  Cys25-Ser147
  Accession # P20096.2
• Specificity: Detects rat IL-4 in ELISAs and Western blots. Does not cross-react with recombinant human IL‑4 or recombinant mouse IL‑4.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4 Antibody (56567) [Alexa Fluor® 488]

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 aa signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.