IL-3R alpha/CD123 Antibody (151231) [PE]

IL-3 dependent myeloid leukemia DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB983P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

IL-3R alpha/CD123 Antibody (151231) [PE] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-3 R alpha /CD123
  Ser17-Lys331
  Accession # P26952
• Specificity: Detects mouse IL-3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-3 R alpha and recombinant mouse IL-3 R beta is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL3RA
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for IL-3R alpha/CD123 Antibody (151231) [PE]

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

Interleukin 3 (IL-3) is a cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hematopoietic cells including pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to a specific cell surface receptor complex. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha  subunit and a beta  subunit. The alpha  subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta . AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha , also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). The alpha subunit is a member of the type I cytokine receptor family, type 5 subfamily (4,5). The mouse IL-3 R alpha extracellular domain (ECD) shares 42% and 29% amino acid sequence identity with rat and human IL-3 R alpha ECD, respectively.

1. Itoh, N. et al. (1990) Science 247:324.
2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
3. Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
4. Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
5. Broughton, S.E. et al. (2015) Cytokine 74:247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3RA
• Uniprot:
  • P26952()