Human IL-33 ELISA Kit (Colorimetric)

Sandwich ELISA: IL-33 ELISA Kit (Colorimetric) [NBP1-84818] - Representative standard curve for human IL-33 ELISA. Human IL-33 was diluted in serial 2-fold steps in CalibratorDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 0.2 pg/mL

Human IL-33 ELISA Kit (Colorimetric) Summary

• Description: The human IL-33 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-33
• Specificity: The assay detects both natural and recombinant human IL-33. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-33 positive sample. No cross reactivity or interference was detected, notably not with IL-1 alpha, IL-1 beta, and IL-18.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 0.2 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.9%
• Intra-Assay: CV% < 4.7%
• Spike Recovery: 99%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL33

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-33 ELISA Kit (Colorimetric)

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 is a member of the IL-1 family of cytokines, which includes IL-1alpha, IL-1 beta, IL-18 and IL-1Ra. In humans, IL-33 mRNA is predominantly found in dermal fibroblasts, bronchial and small airway epithelial cells and smooth muscle cells of skin and lung tissues. This cytokine is localized primarily in the nucleus where it is thought to play a role in transcriptional repression but can also be released from the cell. Upon wounding or infection, IL-33 is released from the cell via an atypical secretory pathway. During apoptosis, IL-33 is cleaved by caspases-3 and 7 (and most likely not caspase-1) to produce an 18-kDa fragment which is released. This cleaved form of IL-33 can bind the ST2 receptor, without subsequent signaling events. Necrosis-mediated release of IL-33 enables binding to ST2, a member of the IL-1 receptor family found on mast and Th2 effector cells, leading to the release of Th2 cytokines. The cytokine production due to IL-33 in turn activates human basophils. IL-33 influence on activation, degranulation, enhanced adhesion and survival of eosinophils has been shown. IL-33 also increases synthesis of IL-6, IL-13, IL-1beta, TNF-alpha, prostaglandin D2 and MCP-1 by primary bone marrow-derived mast cells. Other cells activated by IL-33 are cardiomyocytes, glial cells and CD34+ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL33