Human IL-33 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-33 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-33 Immunoassay is a 4.0 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural... human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.
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• Specificity: Natural and recombinant human IL-33.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-33 Quantikine ELISA Kit

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-33 (D3300B)(31)

Publications using D3300B

• Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N; Endothelial CD34 expression and regulation of immune cell response in-vitro Scientific reports 2023-08-19 [PMID: 37598252] (Human)
• Weng, CM;Lee, MJ;Chao, W;Lin, YR;Chou, CJ;Chen, MC;Chou, CL;Lin Tsai, I;Lin, CH;Fan Chung, K;Kuo, HP; Airway epithelium IgE-Fc?RI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma Mucosal immunology 2023-08-01 [PMID: 37536562] (Human)
• P Szczepanik, M Michalska-, M Kowal, D Krasowska Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea Journal of Clinical Medicine, 2022-10-28;11(21):. 2022-10-28 [PMID: 36362603] (Human)
• W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion Nature Immunology, 2022-07-06;23(7):1021-1030. 2022-07-06 [PMID: 35794369] (Human)
• K Rasheed, U Moens, B Policastro, JI Johnsen, V Koljonen, H Sihto, WO Lui, B Sveinbjørn The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma International Journal of Molecular Sciences, 2022-03-28;23(7):. 2022-03-28 [PMID: 35409061] (Human)
• E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development Journal of Translational Medicine, 2021-12-20;19(1):517. 2021-12-20 [PMID: 34930320] (Human)
• X Zhang, K Bi, X Tu, Q Zhang, Q Cao, Y Liang, P Zeng, L Wang, T Liu, W Fang, H Diao Interleukin-33 as an early predictor of cetuximab treatment efficacy in patients with colorectal cancer Cancer Medicine, 2021-10-19;0(0):. 2021-10-19 [PMID: 34664425] (Human)
• A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis Nature Communications, 2021-10-12;12(1):5947. 2021-10-12 [PMID: 34642338] (Human)
• II Jeican, D Gheban, L Barbu-Tudo, P Ini?ca, C Albu, M Ilie?, S Albu, ML Vic?, HV Matei, S Tripon, M Laz?r, M Alua?, CV Siserman, M Muntean, V Trombitas, CA Iuga, I Opincariu, LM Junie Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33 Journal of Clinical Medicine, 2021-09-12;10(18):. 2021-09-12 [PMID: 34575221] (Human)
• X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence LYSMD3: A mammalian pattern recognition receptor for chitin Cell Reports, 2021-07-20;36(3):109392. 2021-07-20 [PMID: 34289364] (Human)
• H Mado, M Adamczyk-S, W Bartman, K Wierzbicki, B Tadeusiak, P Sowa Plasma Interleukin-33 level in relapsing-remitting multiple sclerosis. Is it negatively correlated with central nervous system lesions in patients with mild disability? Clinical neurology and neurosurgery, 2021-05-20;206(0):106700. 2021-05-20 [PMID: 34030079] (Human)
• W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice J. Immunol., 2020-07-20;0(0):. 2020-07-20 [PMID: 32690653] (Human)
• T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry Allergol Int, 2020-07-08;0(0):. 2020-07-08 [PMID: 32651123] (Human)
• M Saresella, I Marventano, F Piancone, F La Rosa, D Galimberti, C Fenoglio, E Scarpini, M Clerici IL-33 and its decoy sST2 in patients with Alzheimer's disease and mild cognitive impairment J Neuroinflammation, 2020-06-06;17(1):174. 2020-06-06 [PMID: 32505187] (Human)
• P Borsky, Z Fiala, C Andrys, M Beranek, K Hamakova, A Malkova, T Svadlakova, J Krejsek, V Palicka, L Borska, V Rehacek Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris Mediators Inflamm., 2020-04-15;2020(0):8465083. 2020-04-15 [PMID: 32377165] (Human)
• YG Zhuang, YZ Chen, SQ Zhou, H Peng, YQ Chen, DJ Li High plasma levels of pro-inflammatory factors interleukin-17 and interleukin-23 are associated with poor outcome of cardiac-arrest patients: a single center experience BMC Cardiovasc Disord, 2020-04-15;20(1):170. 2020-04-15 [PMID: 32293300] (Human)
• MW Han, SH Kim, I Oh, YH Kim, J Lee Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis Allergy Asthma Clin Immunol, 2019-09-18;15(0):58. 2019-09-18 [PMID: 31548841] (Human)
• AH Al-Aarag, MH Kamel, ER Abdelgawad, SM Abo-Yousse, HH Moussa, ME Elnaggar, RM Hendy, KA Diab Diagnostic role of interleukin -33 in the differentiation of pleural effusions especially tuberculous and malignant effusions BMC Pulm Med, 2019-06-25;19(1):114. 2019-06-25 [PMID: 31238901] (Human)
• N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection Cytokine, 2019-05-29;120(0):258-263. 2019-05-29 [PMID: 31153005] (Human)
• M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa Serum sST2 levels predict severe exacerbation of asthma Respir. Res., 2018-09-03;19(1):169. 2018-09-03 [PMID: 30176857] (Human)
• M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity Cell. Mol. Immunol., 2018-07-20;0(0):. 2018-07-20 [PMID: 30030493] (Human)
• E Rivière, J Sellam, J Pascaud, P Ravaud, JE Gottenberg, X Mariette Serum IL-33 level is associated with auto-antibodies but not with clinical response to biologic agents in rheumatoid arthritis Arthritis Res. Ther., 2018-06-08;20(1):122. 2018-06-08 [PMID: 29884223] (Human)
• ZH Wei, YY Li, SQ Huang, ZQ Tan Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population Cytokine, 2018-04-12;0(0):. 2018-04-12 [PMID: 29656959] (Human)
• F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease Sci Rep, 2017-12-01;7(1):16760. 2017-12-01 [PMID: 29196629] (Human)
• RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris Mediators Inflamm., 2017-02-22;2017(0):7151285. 2017-02-22 [PMID: 28321152] (Human)
• MS Maddur, E Stephen-Vi, M Das, P Prakhar, VK Sharma, V Singh, M Rabin, J Trinath, KN Balaji, F Bolgert, JM Vallat, L Magy, SV Kaveri, J Bayry Regulatory T cell frequency, but not plasma IL-33 levels, represents potential immunological biomarker to predict clinical response to intravenous immunoglobulin therapy J Neuroinflammation, 2017-03-20;14(1):58. 2017-03-20 [PMID: 28320438] (Human)
• JU Lee, HS Chang, HJ Lee, CA Jung, DJ Bae, HJ Song, JS Park, ST Uh, YH Kim, KH Seo, CS Park Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis BMC Pulm Med, 2017-02-15;17(1):39. 2017-02-15 [PMID: 28202030] (Human)
• Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2 Sci Rep, 2016-08-24;6(0):32116. 2016-08-24 [PMID: 27554830] (Human)
• Ketelaar M, Nawijn M, Shaw D, Koppelman G, Sayers I The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma. Clin Exp Allergy, 2016-06-01;46(6):884-7. 2016-06-01 [PMID: 26850082] (Human)
• Bu X, Zhang T, Wang C, Ren T, Wen Z IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production. J Transl Med, 2015-12-16;13(0):381. 2015-12-16 [PMID: 26675669] (Human)
• Guerrero C, Arrieta A, Ramirez N, Rodriguez L, Vega R, Bosch I, Rodriguez J, Narvaez C, Salgado D High plasma levels of soluble ST2 but not its ligand IL-33 is associated with severe forms of pediatric dengue. Cytokine, 2013-01-26;61(3):766-71. 2013-01-26 [PMID: 23357301] (Human)

FAQs for IL-33 (D3300B). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.