Human IL-33 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-33 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-33 Immunoassay is a 4.0 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural... human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.
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• Specificity: Natural and recombinant human IL-33.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-33 Quantikine ELISA Kit

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-33 (D3300B)(31)

Publications using D3300B

• Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N; Endothelial CD34 expression and regulation of immune cell response in-vitro Scientific reports 2023-08-19 [PMID: 37598252] (Human)
• Weng, CM;Lee, MJ;Chao, W;Lin, YR;Chou, CJ;Chen, MC;Chou, CL;Lin Tsai, I;Lin, CH;Fan Chung, K;Kuo, HP; Airway epithelium IgE-Fc?RI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma Mucosal immunology 2023-08-01 [PMID: 37536562] (Human)
• P Szczepanik, M Michalska-, M Kowal, D Krasowska Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea Journal of Clinical Medicine, 2022-10-28;11(21):. 2022-10-28 [PMID: 36362603] (Human)
• W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion Nature Immunology, 2022-07-06;23(7):1021-1030. 2022-07-06 [PMID: 35794369] (Human)
• K Rasheed, U Moens, B Policastro, JI Johnsen, V Koljonen, H Sihto, WO Lui, B Sveinbjørn The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma International Journal of Molecular Sciences, 2022-03-28;23(7):. 2022-03-28 [PMID: 35409061] (Human)
• E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development Journal of Translational Medicine, 2021-12-20;19(1):517. 2021-12-20 [PMID: 34930320] (Human)
• X Zhang, K Bi, X Tu, Q Zhang, Q Cao, Y Liang, P Zeng, L Wang, T Liu, W Fang, H Diao Interleukin-33 as an early predictor of cetuximab treatment efficacy in patients with colorectal cancer Cancer Medicine, 2021-10-19;0(0):. 2021-10-19 [PMID: 34664425] (Human)
• A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis Nature Communications, 2021-10-12;12(1):5947. 2021-10-12 [PMID: 34642338] (Human)
• II Jeican, D Gheban, L Barbu-Tudo, P Ini?ca, C Albu, M Ilie?, S Albu, ML Vic?, HV Matei, S Tripon, M Laz?r, M Alua?, CV Siserman, M Muntean, V Trombitas, CA Iuga, I Opincariu, LM Junie Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33 Journal of Clinical Medicine, 2021-09-12;10(18):. 2021-09-12 [PMID: 34575221] (Human)
• X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence LYSMD3: A mammalian pattern recognition receptor for chitin Cell Reports, 2021-07-20;36(3):109392. 2021-07-20 [PMID: 34289364] (Human)
• H Mado, M Adamczyk-S, W Bartman, K Wierzbicki, B Tadeusiak, P Sowa Plasma Interleukin-33 level in relapsing-remitting multiple sclerosis. Is it negatively correlated with central nervous system lesions in patients with mild disability? Clinical neurology and neurosurgery, 2021-05-20;206(0):106700. 2021-05-20 [PMID: 34030079] (Human)
• W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice J. Immunol., 2020-07-20;0(0):. 2020-07-20 [PMID: 32690653] (Human)
• T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry Allergol Int, 2020-07-08;0(0):. 2020-07-08 [PMID: 32651123] (Human)
• M Saresella, I Marventano, F Piancone, F La Rosa, D Galimberti, C Fenoglio, E Scarpini, M Clerici IL-33 and its decoy sST2 in patients with Alzheimer's disease and mild cognitive impairment J Neuroinflammation, 2020-06-06;17(1):174. 2020-06-06 [PMID: 32505187] (Human)
• P Borsky, Z Fiala, C Andrys, M Beranek, K Hamakova, A Malkova, T Svadlakova, J Krejsek, V Palicka, L Borska, V Rehacek Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris Mediators Inflamm., 2020-04-15;2020(0):8465083. 2020-04-15 [PMID: 32377165] (Human)
• YG Zhuang, YZ Chen, SQ Zhou, H Peng, YQ Chen, DJ Li High plasma levels of pro-inflammatory factors interleukin-17 and interleukin-23 are associated with poor outcome of cardiac-arrest patients: a single center experience BMC Cardiovasc Disord, 2020-04-15;20(1):170. 2020-04-15 [PMID: 32293300] (Human)
• MW Han, SH Kim, I Oh, YH Kim, J Lee Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis Allergy Asthma Clin Immunol, 2019-09-18;15(0):58. 2019-09-18 [PMID: 31548841] (Human)
• AH Al-Aarag, MH Kamel, ER Abdelgawad, SM Abo-Yousse, HH Moussa, ME Elnaggar, RM Hendy, KA Diab Diagnostic role of interleukin -33 in the differentiation of pleural effusions especially tuberculous and malignant effusions BMC Pulm Med, 2019-06-25;19(1):114. 2019-06-25 [PMID: 31238901] (Human)
• N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection Cytokine, 2019-05-29;120(0):258-263. 2019-05-29 [PMID: 31153005] (Human)
• M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa Serum sST2 levels predict severe exacerbation of asthma Respir. Res., 2018-09-03;19(1):169. 2018-09-03 [PMID: 30176857] (Human)
• M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity Cell. Mol. Immunol., 2018-07-20;0(0):. 2018-07-20 [PMID: 30030493] (Human)
• E Rivière, J Sellam, J Pascaud, P Ravaud, JE Gottenberg, X Mariette Serum IL-33 level is associated with auto-antibodies but not with clinical response to biologic agents in rheumatoid arthritis Arthritis Res. Ther., 2018-06-08;20(1):122. 2018-06-08 [PMID: 29884223] (Human)
• ZH Wei, YY Li, SQ Huang, ZQ Tan Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population Cytokine, 2018-04-12;0(0):. 2018-04-12 [PMID: 29656959] (Human)
• F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease Sci Rep, 2017-12-01;7(1):16760. 2017-12-01 [PMID: 29196629] (Human)
• RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris Mediators Inflamm., 2017-02-22;2017(0):7151285. 2017-02-22 [PMID: 28321152] (Human)
• MS Maddur, E Stephen-Vi, M Das, P Prakhar, VK Sharma, V Singh, M Rabin, J Trinath, KN Balaji, F Bolgert, JM Vallat, L Magy, SV Kaveri, J Bayry Regulatory T cell frequency, but not plasma IL-33 levels, represents potential immunological biomarker to predict clinical response to intravenous immunoglobulin therapy J Neuroinflammation, 2017-03-20;14(1):58. 2017-03-20 [PMID: 28320438] (Human)
• JU Lee, HS Chang, HJ Lee, CA Jung, DJ Bae, HJ Song, JS Park, ST Uh, YH Kim, KH Seo, CS Park Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis BMC Pulm Med, 2017-02-15;17(1):39. 2017-02-15 [PMID: 28202030] (Human)
• Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2 Sci Rep, 2016-08-24;6(0):32116. 2016-08-24 [PMID: 27554830] (Human)
• Ketelaar M, Nawijn M, Shaw D, Koppelman G, Sayers I The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma. Clin Exp Allergy, 2016-06-01;46(6):884-7. 2016-06-01 [PMID: 26850082] (Human)
• Bu X, Zhang T, Wang C, Ren T, Wen Z IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production. J Transl Med, 2015-12-16;13(0):381. 2015-12-16 [PMID: 26675669] (Human)
• Guerrero C, Arrieta A, Ramirez N, Rodriguez L, Vega R, Bosch I, Rodriguez J, Narvaez C, Salgado D High plasma levels of soluble ST2 but not its ligand IL-33 is associated with severe forms of pediatric dengue. Cytokine, 2013-01-26;61(3):766-71. 2013-01-26 [PMID: 23357301] (Human)

FAQs for IL-33 (D3300B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.