IL-33 Antibody (IL33305B) - Azide and BSA Free

Western Blot: IL-33 Antibody (IL33305B) [NBP3-11681] - Western blot analysis of human IL-33 using IL-33 antibody (IL33305B) at 1:2,000 dilution.1. Recombinant human IL-33 (His tagged).2. Recombinant human IL-33 (FLAG(R)-tagged).3. Recombinant mouse IL-33 (FLAG(R)-tagged).4. Other protein (His tagged, negative control).

Immunohistochemistry: IL-33 Antibody (IL33305B) [NBP3-11681] - Immunohistochemical staining of human tissue using IL-33 antibody (IL33305B) at 1:100 dilution.A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour).B. Isotype control with IgG2a (negative control).

Immunoprecipitation: IL-33 Antibody (IL33305B) [NBP3-11681] - Immunoprecipitation of recombinant hIL-33 proteins using IL-33 antibody (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP, B/N, ELISA
• Clone: IL33305B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IL-33 Antibody (IL33305B) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IL-33 Antibody (IL33305B) - Azide and BSA Free (NBP3-11681) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human IL-33.
• Specificity: Recognizes human IL-33. Does not cross-react with mouse IL-33.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL33
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Direct ELISA 1:2000-1:5000
  • ELISA 1:2000-1:5000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:2000-1:5000
• Application Notes: Western blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um-filtered solution in PBS, pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for IL-33 Antibody (IL33305B) - Azide and BSA Free

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL33