IL-33 Antibody (6H496)

Western Blot: IL-33 Antibody (6H496) [NBP2-30103] - Analysis of IL-33 using IL-33 monoclonal antibody in (A) HUVEC cell lysate at 10 ug/ml and (B) partial recombinant IL-33 (amino acids 112-270) at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.

Simple Western: IL-33 Antibody (6H496) [NBP2-30103] - Simple Western lane view shows a specific band for IL-33 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

IL-33 Antibody (6H496) Summary

• Immunogen: A partial recombinant human IL-33 (amino acids 112-270) protein was used as immunogen for this antibody.
• Predicted Species: Orangutan (95%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL33
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:50
  • Western Blot 1-10 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IL-33 Antibody (6H496)

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 is a 270 amino acid highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. It is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL33