IL-33 Antibody (40015C) [HRP]

• Applications: WB, IHC, ELISA, ELISA(Cap), ELISA(Det), Flow
• Clone: 40015C
• Clonality: Monoclonal
• Host: Goat
• Conjugate: HRP

IL-33 Antibody (40015C) [HRP] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: E.coli-derived recombinant human IL-33
  Ser112-Thr270
  Accession # O95760
• Specificity: Detects human IL-33 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Goat
• Gene: IL33
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product FAB36253. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from cell culture supernatant

Alternate Names for IL-33 Antibody (40015C) [HRP]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL33