IL-32 Antibody (373821) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 APC-conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 373821
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

IL-32 Antibody (373821) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human IL-32 alpha
  Met1-Asn131
  Accession # P24001
• Specificity: Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL32
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-32 Antibody (373821) [Allophycocyanin]

• IL32
• IL-32
• IL-32alpha
• IL-32beta
• interleukin 32
• interleukin-32 theta
• interleukin-32
• natural killer cell transcript 4
• Natural killer cells protein 4
• NK4
• NK4IL-32delta
• TAIF
• TAIFa
• TAIFb
• TAIFc
• TAIFd
• TAIFIL-32gamma
• Tumor necrosis factor alpha-inducing factor

Background

IL-32 alpha is one of at least nine potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4), which when full-length is 31 kDa in size. The predicted molecular weight of IL-32 alpha is approximately 15 kDa and it represents amino acids (aa) 1-18, 65-153, and 211-234 of the full-length (234 aa) form. Since the signal sequence of the ORF is aa 1-30, this isofom is like found intracellularly. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-32 Antibody (IC30402A)(5)

Publications using IC30402A

• KP Devalraju, VSK Neela, SS Krovvidi, R Vankayalap, VL Valluri Defective expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection PLoS ONE, 2021-09-13;16(9):e0257185. 2021-09-13 [PMID: 34516566] (Flow Cytometry, Human)
• Qiuming Yao, Bin Wang, Xi Jia, Qian Li, Wei Yao, Jin-an Zhang Increased Human Interleukin-32 Expression Is Related to Disease Activity of Graves' Disease Front Endocrinol (Lausanne) 9/26/2019 [PMID: 31616372]
• Henna Kallionpää, Juhi Somani, Soile Tuomela, Ubaid Ullah, Rafael de Albuquerque, Tapio Lönnberg et al. Early Detection of Peripheral Blood Cell Signature in Children Developing beta -Cell Autoimmunity at a Young Age Diabetes 10/1/2019 [PMID: 31311800]
• Pedro M M Mesquita Role of IL-32 in HIV Reactivation and its Link to HIV-HSV Coinfection J. Infect. Dis, 2017-02-15;0(0):. 2017-02-15 [PMID: 28007920] (Flow Cytometry, Human)
• Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure. PLoS ONE, 2015-08-04;10(8):e0134568. 2015-08-04 [PMID: 26241657] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL32
• Uniprot:
  • P24001()