Recombinant Human IL-31 Animal-Free Protein

Bioactivity: Recombinant Human IL-31 Animal-Free Protein [NBP2-76195] - IL-31 induced activation of STAT3 in U-87MG cells.

Product Discontinued

Recombinant Human IL-31 Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: IL-31 is a monomer, 15.8 kDa (141 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: STAT3 Activation in U-87 MG cells, (ED50 acceptance criteria are < 25 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL31
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 15.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Human IL-31 Animal-Free Protein

• IL31
• IL-31
• IL-31interleukin-31
• interleukin 31

Background

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting ofIL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma(Dillon et al., 2004 (PubMed 15184896)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL31