Recombinant Human IL-31 Protein

SDS-Page: Recombinant Human IL-31 Protein [NBP2-34870] - SDS-PAGE with Recombinant human IL-31 protein.

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-31 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 141 amino acids corresponding to IL-31 Source: E. coli

  Uniprot ID: Q6EBC2

  Amino Acid Sequence: SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T

• Details of Functionality: IL-31 protein is fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of IL-31 can effectively induce STAT3 activation
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL31
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-31 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-31 Protein

• IL31
• IL-31
• IL-31interleukin-31
• interleukin 31

Background

Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares 24 % a.a. sequence identity in the mature protein with mouse IL-31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL31
• Uniprot:
  • Q6EBC2()
  • Q6EBC2()
  • Q6EBC2()