Human IL-31 ELISA Kit (Colorimetric)

ELISA: IL-31 ELISA Kit [NBP1-83745] - Standard curve

Product Discontinued

Human IL-31 ELISA Kit (Colorimetric) Summary

• Specificity: The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-31 positive sample. There was no cross reactivity or interference detected.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 1.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 2.9%
• Intra-Assay: CV% < 3.6%
• Spike Recovery: The unspiked serum was used as blank in these experiments. For recovery data see the Manual.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL31

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-31 ELISA Kit (Colorimetric)

• IL31
• IL-31
• IL-31interleukin-31
• interleukin 31

Background

IL-31 is a 24kD and 164 AA alpha-helical protein with a four-helix bundle structure, preferentially produced by CD 4+ type 2 helper T cells. IL-31 is closely related to the IL-6 type cytokines such as oncostatin M, Leukemia inhibitory factor and cardiotrophin 1. IL-31 signals via binding to a heterodimeric complex that is composed of IL-31 receptor A (IL-31RA) that is highly homologous with the gp 30-like receptor and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes, in epithelial cells, and keratinocytes respectively. On target cells IL-31 binding can induce activation of the JAK/STAT, AKT and MAPK signaling pathways. Due to the ubiquitous expression of its receptor complex, IL-31 has numerous physiological roles including regulation of hematopoiesis and immune response. When overexpressed in transgenic mice, IL-31 induces severe pruritus resembling eczema in humans. Serum IL-31 was previously found overexpressed in adults with atopic dermatitis (AD). Serum measurement of IL-31 in paediatric atopic dermatitis revealed that elevated levels correlate with severity scoring. Activated effector T cells are recruited to the skin by chemokines and cell adhesion molecules. These components in the local microenvironment prolong T cell survival and contribute to the chronicity of these diseases. IL-31 is associated with the promotion of allergic and chronic inflammatory conditions such as dermatitis, pruritus, airway hypersensititvity and inflammatory bowel disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-31 ELISA Kit (NBP1-83745). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL31