Human IL-31 ELISA Kit (Colorimetric) Summary
The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-31 positive sample. There was no cross reactivity or interference detected.
|Standard Curve Range
7.8 - 500 pg/mL
The unspiked serum was used as blank in these experiments.
For recovery data see the Manual.
ELISA Kit (Colorimetric)
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Human IL-31 Standard lyophilized, 500 pg/ml upon reconstitution
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Biotin-Conjugate anti-human IL-31 polyclonal antibody
- Calibrator Diluent
- Conjugate Diluent
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-31
Alternate Names for Human IL-31 ELISA Kit (Colorimetric)
- interleukin 31
IL-31 is a 24kD and 164 AA alpha-helical protein with a four-helix bundle structure, preferentially produced by CD 4+ type 2 helper T cells. IL-31 is closely related to the IL-6 type cytokines such as oncostatin M, Leukemia inhibitory factor and cardiotrophin 1. IL-31 signals via binding to a heterodimeric complex that is composed of IL-31 receptor A (IL-31RA) that is highly homologous with the gp 30-like receptor and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes, in epithelial cells, and keratinocytes respectively. On target cells IL-31 binding can induce activation of the JAK/STAT, AKT and MAPK signaling pathways. Due to the ubiquitous expression of its receptor complex, IL-31 has numerous physiological roles including regulation of hematopoiesis and immune response. When overexpressed in transgenic mice, IL-31 induces severe pruritus resembling eczema in humans. Serum IL-31 was previously found overexpressed in adults with atopic dermatitis (AD). Serum measurement of IL-31 in paediatric atopic dermatitis revealed that elevated levels correlate with severity scoring. Activated effector T cells are recruited to the skin by chemokines and cell adhesion molecules. These components in the local microenvironment prolong T cell survival and contribute to the chronicity of these diseases. IL-31 is associated with the promotion of allergic and chronic inflammatory conditions such as dermatitis, pruritus, airway hypersensititvity and inflammatory bowel disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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