Human IL-31 ELISA Kit (Colorimetric)


ELISA: IL-31 ELISA Kit [NBP1-83745] - Standard curve

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
7.8 - 500 pg/mL
1.0 pg/mL

Order Details

Human IL-31 ELISA Kit (Colorimetric) Summary

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-31 positive sample. There was no cross reactivity or interference detected.
Standard Curve Range
7.8 - 500 pg/mL
1.0 pg/mL
Assay Type
Sandwich ELISA
Spike Recovery
The unspiked serum was used as blank in these experiments. For recovery data see the Manual.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Substrate Solution (tetramethyl-benzidine)
  2. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  3. Human IL-31 Standard lyophilized, 500 pg/ml upon reconstitution
  4. Streptavidin-HRP
  5. Stop Solution (1M Phosphoric acid)
  6. Sample Diluent
  7. Biotin-Conjugate anti-human IL-31 polyclonal antibody
  8. Calibrator Diluent
  9. Conjugate Diluent
  10. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  11. Adhesive Films
  12. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-31

Alternate Names for Human IL-31 ELISA Kit (Colorimetric)

  • IL31
  • IL-31
  • IL-31interleukin-31
  • interleukin 31


IL-31 is a 24kD and 164 AA alpha-helical protein with a four-helix bundle structure, preferentially produced by CD 4+ type 2 helper T cells. IL-31 is closely related to the IL-6 type cytokines such as oncostatin M, Leukemia inhibitory factor and cardiotrophin 1. IL-31 signals via binding to a heterodimeric complex that is composed of IL-31 receptor A (IL-31RA) that is highly homologous with the gp 30-like receptor and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes, in epithelial cells, and keratinocytes respectively. On target cells IL-31 binding can induce activation of the JAK/STAT, AKT and MAPK signaling pathways. Due to the ubiquitous expression of its receptor complex, IL-31 has numerous physiological roles including regulation of hematopoiesis and immune response. When overexpressed in transgenic mice, IL-31 induces severe pruritus resembling eczema in humans. Serum IL-31 was previously found overexpressed in adults with atopic dermatitis (AD). Serum measurement of IL-31 in paediatric atopic dermatitis revealed that elevated levels correlate with severity scoring. Activated effector T cells are recruited to the skin by chemokines and cell adhesion molecules. These components in the local microenvironment prolong T cell survival and contribute to the chronicity of these diseases. IL-31 is associated with the promotion of allergic and chronic inflammatory conditions such as dermatitis, pruritus, airway hypersensititvity and inflammatory bowel disease.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
Species: Mu
Applications: WB, IHC
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Neut
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD

Publications for IL-31 ELISA Kit (NBP1-83745) (0)

There are no publications for IL-31 ELISA Kit (NBP1-83745).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-31 ELISA Kit (NBP1-83745) (0)

There are no reviews for IL-31 ELISA Kit (NBP1-83745). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-31 ELISA Kit (NBP1-83745) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-31 Products

Bioinformatics Tool for IL-31 ELISA Kit (NBP1-83745)

Discover related pathways, diseases and genes to IL-31 ELISA Kit (NBP1-83745). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-31 ELISA Kit (NBP1-83745)

Discover more about diseases related to IL-31 ELISA Kit (NBP1-83745).

Pathways for IL-31 ELISA Kit (NBP1-83745)

View related products by pathway.

PTMs for IL-31 ELISA Kit (NBP1-83745)

Learn more about PTMs related to IL-31 ELISA Kit (NBP1-83745).

Blogs on IL-31

There are no specific blogs for IL-31, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IL-31 ELISA Kit (Colorimetric) and receive a gift card or discount.


Gene Symbol IL31