Human IL-31 ELISA Kit (Colorimetric)

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ELISA: IL-31 ELISA Kit [NBP1-83745] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
1.0 pg/mL

Order Details

Human IL-31 ELISA Kit (Colorimetric) Summary

Specificity
The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-31 positive sample. There was no cross reactivity or interference detected.
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
1.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
2.9%
Intra-Assay
3.6%
Spike Recovery
The unspiked serum was used as blank in these experiments. For recovery data see the Manual.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL31

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  2. Streptavidin-HRP
  3. Substrate Solution (tetramethyl-benzidine)
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Biotin-Conjugate anti-human IL-31 polyclonal antibody
  7. Calibrator Diluent
  8. Conjugate Diluent
  9. Human IL-31 Standard lyophilized, 500 pg/ml upon reconstitution
  10. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  11. Adhesive Films
  12. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-31

Alternate Names for Human IL-31 ELISA Kit (Colorimetric)

  • IL31
  • IL-31
  • IL-31interleukin-31
  • interleukin 31

Background

IL-31 is a 24kD and 164 AA alpha-helical protein with a four-helix bundle structure, preferentially produced by CD 4+ type 2 helper T cells. IL-31 is closely related to the IL-6 type cytokines such as oncostatin M, Leukemia inhibitory factor and cardiotrophin 1. IL-31 signals via binding to a heterodimeric complex that is composed of IL-31 receptor A (IL-31RA) that is highly homologous with the gp 30-like receptor and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes, in epithelial cells, and keratinocytes respectively. On target cells IL-31 binding can induce activation of the JAK/STAT, AKT and MAPK signaling pathways. Due to the ubiquitous expression of its receptor complex, IL-31 has numerous physiological roles including regulation of hematopoiesis and immune response. When overexpressed in transgenic mice, IL-31 induces severe pruritus resembling eczema in humans. Serum IL-31 was previously found overexpressed in adults with atopic dermatitis (AD). Serum measurement of IL-31 in paediatric atopic dermatitis revealed that elevated levels correlate with severity scoring. Activated effector T cells are recruited to the skin by chemokines and cell adhesion molecules. These components in the local microenvironment prolong T cell survival and contribute to the chronicity of these diseases. IL-31 is associated with the promotion of allergic and chronic inflammatory conditions such as dermatitis, pruritus, airway hypersensititvity and inflammatory bowel disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL31
Entrez