IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - IHC-P detection of IL27RA protein in a section of normal human colon tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. Intense membrane with cytoplasmic staining was observed in the cells of crypts and the absorptive columnar epithelium

Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-19015. Surface staining of IL27R on freshly isolated (PPI negative) human PBMCs using IL-27R antibody and CD3 antibody at 10 ul/10^6 cells. Flow kit, mouse IgG2b isotype control antibody, and RBC lysis buffer were used for this test.

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - Adenocarcinoma of the stomach probed with IL-27R antibody at 5 ug/ml. Image from the standard format of this antibody.

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - FFPE human prostate probed with IL-27R antibody at 5 ug/ml. Image from the standard format of this antibody.

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - IHC-P detection of IL27RA protein in a section of human colo-rectal cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed specific positivity for IL27RA and the staining intensity was more towards the cellular

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - IHC-P detection of IL27RA protein in a section of human renal/kidney cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The representative image shows membrane-cytoplasmic positivity for IL27RA protein in the cancer cells. Image f

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - IHC-P detection of IL27RA protein in a section of human stomach cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed intense membrane-cytoplasmic positivity for IL27RA protein, whereas, the glands in adjacent

Flow Cytometry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - Surface staining on human peripheral blood monocytes using IL-27R antibody at 1 ug/10^6 cells (red represents specific antibody and green represents isotype control). APC-conjugated goat anti-mouse Ig F(ab')2 secondary antibody , CS flow kit and an FcR block were used for this test. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 34N4G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free (NBP2-19015) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 200-250 of human IL-27R (WSX-1) was used as the immunogen for this antibody.
• Localization: Membrane (with Extracellular and Cytoplasmic domains)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL27RA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface) 1 ug/ml
  • Flow Cytometry 1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

• class I cytokine receptor
• CRL1IL-27R
• Cytokine receptor-like 1
• IL-27 R alpha
• IL27R alpha
• IL27R
• IL27RA
• IL-27Ra
• IL-27R-alpha
• interleukin 27 receptor, alpha
• interleukin-27 receptor subunit alpha
• TCCR
• TCCRIL-27 receptor subunit alpha
• T-cell cytokine receptor type 1
• Type I T-cell cytokine receptor
• WSX-1
• WSX1IL-27R subunit alpha
• zcytor1

Background

The IL-27 Receptor complex includes WSX-1 and gp130. IL-27R (WSX-1) is 65-70 kDa protein and is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes including CD4+ and CD8+ T cells, monocytes, DCs and NK cells (1-3). IL-27R activation includes STAT1, STAT3, STAT4 and STAT5, leading to differing pathway dependent effector activities. Like the IL12/IL-12R complex, the IL-27/IL-27R complex also has Th1 enhancing activity especially enhancement of IFNg production by naive T cells and NK cells (1). IL-27R mediates downregulation of Th2 activity by findings that IL-27R deficiencies mitigate inflammatory pathology in helminthic infections in mice (1, 4, 5). The possibility of utilizing IL-27R as a target for autoimmune inflammatory diseases, especially of mucosal surfaces as in human IBD, is under investigation, measured by Th2 type cytokines and bolstered through Th2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL27RA