Mouse IL-23 ELISA Kit (Colorimetric)

Mouse IL-23 R ELISA Kit [NBP1-92664] - Data for Mouse IL-23 Platinum ELISA

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (citrate), buffered solution
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 16.0 pg/mL

Mouse IL-23 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The assay detects both natural and recombinant mouse IL-23. The interference of GM-CSF, IFN gamma, IL-1 alpha, IL-2, IL-4, IL-5, IL- 6, IL-10, IL-12p70, IL-13, IL-17, IL-18, IL-22, TNF alpha, MCP-1, MCP-3, MIP-1 alpha, MIP-1 beta, RANTES, IP-10 was evaluated by spiking these proteins at physiologically relevant concentrations into IL-23 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 16.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 2.50%
• Intra-Assay: CV% < 8.30%
• Spike Recovery: The unspiked serum was used as blank in these experiments. The recovery ranged from 41% to 53% with an overall mean recovery of 49%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL23A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-23 ELISA Kit (Colorimetric)

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12B
• IL-23 subunit alpha
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL23P19P19
• interleukin 12, p40
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-12 beta chain
• interleukin-12 subunit beta
• Interleukin-23 subunit p19
• interleukin-six, G-CSF related factor
• JKA3 induced upon T-cell activation
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• P19
• SGRF

Background

IL-23 (Interleukin-23) is a heterodimeric cytokine consisting of two subunits, p19 and p40. IL-23 plays an important role in the inflammatory response against infection. It is a key element in the pathogenesis of inflammatory bowel disease, which comprises Crohn's disease and ulcerative colitis. It can also have implications in the development of cancerous tumors. IL-23 promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T cell infiltration. In conjunction with IL-6 and TGF-b1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-a, NOS-2, and chemokines resulting in inflammation (McGovern D et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-23 ELISA Kit (NBP1-92664). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL23A