IL-23R Antibody (218213) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) (A) cultured with plate-bound 5 μg/mL anti-Human CD3, 2 μg/mL anti-Human CD28 (AF-342-PB), 20 ng/mL Recombinant Human IL-2 (202-GMP), 10 ng/mL TGF-beta (100-B), 20 ng/mL Recombinant Human IL-23 (1290-IL), 40 ng/mL Recombinant Human IL-6 (206-IL), 10 ng/mL Recombinant Human IL-1 beta (201-LB), and 10 μg/mL anti-Human IFN-gamma (AF-285-NA) for 7 days to induce Th17 development or (B) resting, were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and Mouse Anti-Human IL-23 R APC-conjugated Monoclonal Antibody (Catalog # FAB14001A). Quadrant markers were set based on Mouse IgG2B Isotype Control (IC0041A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Product Discontinued

IL-23R Antibody (218213) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-23 R
  Gly24-Ile354
  Accession # Q5VWK5
• Specificity: Detects human IL-23 R in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑23 R.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL23R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23R Antibody (218213) [Allophycocyanin]

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5448.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23R Antibody (FAB14001A)(4)

Publications using FAB14001A

• HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis Nat Commun, 2019-02-12;10(1):709. 2019-02-12 [PMID: 30755603] (Flow Cytometry, Human)
• Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis. J Immunol, 2015-04-20;194(11):5139-49. 2015-04-20 [PMID: 25895532] (Flow Cytometry, Human)
• Shen H, Zhang W, Abraham C, Cho J Age and CD161 expression contribute to inter-individual variation in interleukin-23 response in CD8+ memory human T cells. PLoS ONE, 2013-03-01;8(3):e57746. 2013-03-01 [PMID: 23469228] (Flow Cytometry, Human)
• Madrid , Alexis S, Ganem , Don Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol, 2012-06-06;86(16):8693-704. 2012-06-06 [PMID: 22674989] (Flow Cytometry, Human)

Reviews for IL-23R Antibody (FAB14001A) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24549990
• Species: Human

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24549990
• Species: Human

Bioinformatics

• Gene Symbol: IL23R
• Uniprot:
  • Q5VWK5()