Mouse IL-23 ELISA Kit (Colorimetric)

ELISA: Mouse IL-23 ELISA Kit (Colorimetric) [NBP2-31050] - Standard curve using the IL-23 standard.

Product Discontinued

Mouse IL-23 ELISA Kit (Colorimetric) Summary

• Specificity: Measures free mouse IL-23 in the supernatant of activated cells.
• Standard Curve Range: 31.25-1000 pg/ml
• Sensitivity: 8 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL23A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The Mouse IL-23 ActivELISA(TM) Kit measures free IL-23 in the supernatant of activated cells. Standard protocols for detecting IL-23 activity include ELISA and Western Blot. The IL-23 ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-p19 antibody is coated on a plate which then captures free IL-23. The amount of bound IL-23 is detected by adding a second biotinylated anti-p40 antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

The IL-12/IL-23 standard protein was produced in insect cells infected with baculovirus.

Alternate Names for Mouse IL-23 ELISA Kit (Colorimetric)

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12B
• IL-23 subunit alpha
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL23P19P19
• interleukin 12, p40
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-12 beta chain
• interleukin-12 subunit beta
• Interleukin-23 subunit p19
• interleukin-six, G-CSF related factor
• JKA3 induced upon T-cell activation
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• P19
• SGRF

Background

IL-23 (Interleukin-23) is a heterodimeric cytokine consisting of two subunits, p19 and p40. IL-23 plays an important role in the inflammatory response against infection. It is a key element in the pathogenesis of inflammatory bowel disease, which comprises Crohn's disease and ulcerative colitis. It can also have implications in the development of cancerous tumors. IL-23 promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T cell infiltration. In conjunction with IL-6 and TGF-b1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-a, NOS-2, and chemokines resulting in inflammation (McGovern D et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-23 ELISA Kit (NBP2-31050)(2)

Publications using NBP2-31050

• Vossenkamper A, Marches O, Fairclough PD et al. Inhibition of NF-kB signaling in human dendritic cells by the enteropathogenic Escherichia coli effector protein NleE. J Immunol. 2010-10-01 [PMID: 20833837] (Human)
  
  Details:
  Nuclear and cytosolic extracts from monocyte derived human dendritic cells in the presence of EPEC LPS, Fig 3D.
• Kondo A, Yamashita T, Tamura H et al. Interferon-gamma and tumor necrosis factor-alpha induce an immunoinhibitory molecule, B7-H1, via nuclear factor-kappaB activation in blasts in myelodysplastic syndromes. Blood. 2010-08-19 [PMID: 20472834]
  
  Details:
  NF-kB/p65 ActivELISA (IMK-503) kit and Nuclear extraction kit (10081K). SKM-1 leukemic cells were separated into cytoplasmic and nuclear fractions then subject to western blot analysis, Fig 2A &B.

FAQs for IL-23 ELISA Kit (NBP2-31050). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL23A