Mouse IL-23 ELISA Kit (Colorimetric)

ELISA: Mouse IL-23 ELISA Kit (Colorimetric) [NBP2-31050] - Standard curve using the IL-23 standard.

Product Discontinued

Mouse IL-23 ELISA Kit (Colorimetric) Summary

• Specificity: Measures free mouse IL-23 in the supernatant of activated cells.
• Standard Curve Range: 31.25-1000 pg/ml
• Sensitivity: 8 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL23A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The Mouse IL-23 ActivELISA(TM) Kit measures free IL-23 in the supernatant of activated cells. Standard protocols for detecting IL-23 activity include ELISA and Western Blot. The IL-23 ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-p19 antibody is coated on a plate which then captures free IL-23. The amount of bound IL-23 is detected by adding a second biotinylated anti-p40 antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

The IL-12/IL-23 standard protein was produced in insect cells infected with baculovirus.

Alternate Names for Mouse IL-23 ELISA Kit (Colorimetric)

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12B
• IL-23 subunit alpha
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL23P19P19
• interleukin 12, p40
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-12 beta chain
• interleukin-12 subunit beta
• Interleukin-23 subunit p19
• interleukin-six, G-CSF related factor
• JKA3 induced upon T-cell activation
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• P19
• SGRF

Background

IL-23 (Interleukin-23) is a heterodimeric cytokine consisting of two subunits, p19 and p40. IL-23 plays an important role in the inflammatory response against infection. It is a key element in the pathogenesis of inflammatory bowel disease, which comprises Crohn's disease and ulcerative colitis. It can also have implications in the development of cancerous tumors. IL-23 promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T cell infiltration. In conjunction with IL-6 and TGF-b1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-a, NOS-2, and chemokines resulting in inflammation (McGovern D et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-23 ELISA Kit (NBP2-31050)(2)

Publications using NBP2-31050

• Vossenkamper A, Marches O, Fairclough PD et al. Inhibition of NF-kB signaling in human dendritic cells by the enteropathogenic Escherichia coli effector protein NleE. J Immunol. 2010-10-01 [PMID: 20833837] (Human)
  
  Details:
  Nuclear and cytosolic extracts from monocyte derived human dendritic cells in the presence of EPEC LPS, Fig 3D.
• Kondo A, Yamashita T, Tamura H et al. Interferon-gamma and tumor necrosis factor-alpha induce an immunoinhibitory molecule, B7-H1, via nuclear factor-kappaB activation in blasts in myelodysplastic syndromes. Blood. 2010-08-19 [PMID: 20472834]
  
  Details:
  NF-kB/p65 ActivELISA (IMK-503) kit and Nuclear extraction kit (10081K). SKM-1 leukemic cells were separated into cytoplasmic and nuclear fractions then subject to western blot analysis, Fig 2A &B.

FAQs for IL-23 ELISA Kit (NBP2-31050). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL23A