Mouse IL-23 ELISA Kit (Colorimetric)

Mouse IL-23 R ELISA Kit [NBP1-92664] - Data for Mouse IL-23 Platinum ELISA

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (citrate), buffered solution
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 16.0 pg/mL

Mouse IL-23 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The assay detects both natural and recombinant mouse IL-23. The interference of GM-CSF, IFN gamma, IL-1 alpha, IL-2, IL-4, IL-5, IL- 6, IL-10, IL-12p70, IL-13, IL-17, IL-18, IL-22, TNF alpha, MCP-1, MCP-3, MIP-1 alpha, MIP-1 beta, RANTES, IP-10 was evaluated by spiking these proteins at physiologically relevant concentrations into IL-23 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 16.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 2.50%
• Intra-Assay: CV% < 8.30%
• Spike Recovery: The unspiked serum was used as blank in these experiments. The recovery ranged from 41% to 53% with an overall mean recovery of 49%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL23A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-23 ELISA Kit (Colorimetric)

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12B
• IL-23 subunit alpha
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL23P19P19
• interleukin 12, p40
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-12 beta chain
• interleukin-12 subunit beta
• Interleukin-23 subunit p19
• interleukin-six, G-CSF related factor
• JKA3 induced upon T-cell activation
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• P19
• SGRF

Background

IL-23 (Interleukin-23) is a heterodimeric cytokine consisting of two subunits, p19 and p40. IL-23 plays an important role in the inflammatory response against infection. It is a key element in the pathogenesis of inflammatory bowel disease, which comprises Crohn's disease and ulcerative colitis. It can also have implications in the development of cancerous tumors. IL-23 promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T cell infiltration. In conjunction with IL-6 and TGF-b1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-a, NOS-2, and chemokines resulting in inflammation (McGovern D et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL23A