IL-21R Antibody (155516) [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB
• Clone: 155516
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

IL-21R Antibody (155516) [Alexa Fluor® 700] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-21 R
  Cys20-Pro236
  Accession # Q9JHX3
• Specificity: Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-21 R or recombinant mouse IL-2 R beta is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-21R Antibody (155516) [Alexa Fluor® 700]

• CD360 antigen
• CD360
• IL-21 R
• IL-21 receptor
• IL21R
• IL-21R
• interleukin 21 receptor
• interleukin-21 receptor
• MGC10967
• NILR
• Novel interleukin receptor

Background

IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1-5). Complex formation between IL-21 R and the common gamma  chain ( gamma _c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL-21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL‑21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3-5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10).  IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8⁺ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.