Recombinant Mouse IL-21 Animal-Free Protein

SDS-Page: Recombinant Mouse IL-21 Animal-Free Protein [NBP2-78045] - 1 ug run under (-) non-reducing and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Mouse IL-21 Animal-Free Protein [NBP2-78045] - Induced proliferation of B9 cells.

Bioactivity: Recombinant Mouse IL-21 Animal-Free Protein [NBP2-78045] - Induced proliferation of primary mouse thymocytes.

Product Discontinued

Recombinant Mouse IL-21 Animal-Free Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: IL-21 is a monomer, 15.1 kDa (130 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: Mouse thymocyte proliferation with costimulation or proliferation of B9 cells, >5 x 10^4 units/mg (ED50 acceptance criteria are <20 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL21
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 15.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 8.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Mouse IL-21 Animal-Free Protein

• CVID11
• IL21
• IL-21
• IL-21Za11interleukin-21
• interleukin 21
• interleukin-21 isoform
• Za11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL21