Recombinant Human IL-2 [ Cys Ser125]Protein

SDS-Page: Recombinant Human IL-2 [ Cys Ser125]Protein [NBP3-07100] - Western blot under reducing and non-reducing conditions

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Concentration: LYOPH

Recombinant Human IL-2 [ Cys Ser125]Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 133 amino acids corresponding to IL-2 Source: E. coli

  Amino Acid Sequence: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT

• Modification: Cys Ser125
• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1.0 EU/ug of IL-2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB (pH 3.5).
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL.

Alternate Names for Recombinant Human IL-2 [ Cys Ser125]Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL2