Recombinant Human IL-2 Protein

SDS-Page: Human IL-2 Protein [NBP2-35054]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 133 amino acids corresponding to IL-2 Source: E. coli

  Uniprot ID: P60568

  Amino Acid Sequence: APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

• Details of Functionality: IL2 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1.0 EU/ug of IL-2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 3.5.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water to a concentration of 0.1-1.0mg/mL. Do not reconstitute in cell culture media directly.

Alternate Names for Recombinant Human IL-2 Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required forT-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2R alpha, 75 kDa IL-2R beta, and 64 kDa common gamma chain gammac/IL-2Rgamma) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56 % and 66 % amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IL-2 Recombinant Protein (NBP2-35054)(4)

Publications using NBP2-35054

• Devadas S iCa2+ Flux, ROS and IL-10 Determines Cytotoxic, and Suppressor T Cell Functions in Chronic Human Viral Infections Front Immunol 2020-03-06 [PMID: 32210950]
• Zhaoxu Li, Junzhe Zhang, Jicun Tang, Ruiying Wang. Celastrol increases osteosarcoma cell lysis by gdT cells through up-regulation of death receptors. Oncotarget 2016-10-22 [PMID: 27768597]
• Zhaoxu Li, Huiqin Peng, Qiang Xu, Zhaoming Ye. Sensitization of human osteosarcoma cells to Vg9Vdelta2 T-cell-mediated cytotoxicity by zoledronate. J Orthop Res 2012-05-01 [PMID: 22025284]
• Zhaoxu Li, Qiang Xu, Huiqin Peng et al. IFN-g enhances HOS and U2OS cell lines susceptibility to gdT cell-mediated killing through the Fas/Fas ligand pathway. Int Immunopharmacol 2011-04-01 [PMID: 21238618]

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P60568()
  • P60568()
  • P60568()