IL-2 R beta Antibody (TU27) - Low Endotoxin

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104] - Separation of human CD122 positive CD56 positive CD3 negative NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD122 (TU27) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104] - Surface staining pattern of human peripheral whole blood stained using anti-human CD122 (TU27) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104] - Multicolor surface staining of human CD3 negative lymphocytes stained using anti-human CD122 (TU27) purified antibody (concentration in sample 4 ug/ml, GAM APC) and anti-human CD56 (LT56) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).

• Reactivity: Hu
• Applications: Flow, Func, IP
• Clone: TU27
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Low Endotoxin
• Concentration: 1 mg/ml

IL-2 R beta Antibody (TU27) - Low Endotoxin Summary

• Additional Information: Clone TU27 was used by HLDA to establish CD designation.
• Immunogen: TL-Mor cell line
• Specificity: The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RB
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Functional
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.2 um filter sterilized
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IL-2 R beta Antibody (TU27) - Low Endotoxin

• CD122 antigen
• CD122
• high affinity IL-2 receptor beta subunit
• High affinity IL-2 receptor subunit beta
• IL-15 R beta
• IL-2 R beta
• IL-2 receptor subunit beta
• IL2R beta
• IL-2R subunit beta
• IL2RB
• IL-2Rb
• interleukin 15 receptor, beta
• interleukin 2 receptor, beta
• interleukin-2 receptor subunit beta
• P70-75
• p75

Background

The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RB