IL-2 R beta Antibody (TM-b1) - BSA Free

Flow Cytometry: IL-2 R beta Antibody (TM-b1) [NBP1-43355] - Staining of C57Bl/6 splenocytes with Anti-Mouse NK1.1 APC and 0.25 ug of Rat IgG2b K Isotype Control Purified (left) or 0.5 ug of Anti-Mouse CD122 Purified (right) followed by Anti-Rat IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.

• Reactivity: Mu
• Applications: Flow, IP, B/N
• Clone: TM-b1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

IL-2 R beta Antibody (TM-b1) - BSA Free Summary

• Immunogen: The immunogen for this antibody was Purified CD122.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2RB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
• Application Notes: The TM-beta1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-2 R beta Antibody (TM-b1) - BSA Free

• CD122 antigen
• CD122
• high affinity IL-2 receptor beta subunit
• High affinity IL-2 receptor subunit beta
• IL-15 R beta
• IL-2 R beta
• IL-2 receptor subunit beta
• IL2R beta
• IL-2R subunit beta
• IL2RB
• IL-2Rb
• interleukin 15 receptor, beta
• interleukin 2 receptor, beta
• interleukin-2 receptor subunit beta
• P70-75
• p75

Background

The TM-beta1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain (IL-2Rbeta). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-beta1 antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RB