Non-human Primate IL-2 ELISA Kit

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ELISA: Non-human Primate IL-2 ELISA kit [NBP3-11727] - Representative standard curve.

Product Details

Summary
Reactivity Pm-NHPSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell culture supernatants
Standard Curve Range
3.16 - 1000 pg/mL
Sensitivity
1 pg/mL

Order Details

Non-human Primate IL-2 ELISA Kit Summary

Standard Curve Range
3.16 - 1000 pg/mL
Sensitivity
1 pg/mL
Inter-Assay
%CV < 6.0
Intra-Assay
%CV < 3.7
Spike Recovery
71 - 106%
Sample Volume
100 uL
Kit Type
ELISA Kit
Gene
IL2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated detection mAb (MT8G10)
  2. Buffers for washing and dilution and standard reconstitution
  3. Pre-coated ELISA strip plates (mAbs MT2A91/2C95)
  4. Recombinant human IL-2 ELISA standard
  5. Streptavidin-HRP
  6. Substrate (TMB)

Alternate Names for Non-human Primate IL-2 ELISA Kit

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • Proleukin
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IL-2 ELISA Kit (NBP3-11727). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol IL2