Rat IL-2 ELISA Kit (Colorimetric)

ELISA: Rat IL-2 ELISA Kit (Colorimetric) [NBP1-92704]

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 62.5 - 2,000 pg/mL
• Sensitivity: 11.0 pg/mL

Rat IL-2 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-2 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 62.5 - 2,000 pg/mL
• Sensitivity: 11.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: The unspiked serum was used as blank in these experiments. The overall mean recovery was 81%.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IL-2 ELISA Kit (Colorimetric)

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors in an autocrine fashion on the surface of T cells. Apart from its most important role to mediate antigen specific T lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B and NK cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T cells to synthesize IL-2 have been observed in physiologic and pathologic states.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL2