IL-2 Antibody [Biotin]

IL-2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1890) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Fe
• Applications: WB, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant feline IL‑2
  Ala21-Thr154 (Cys146Ser)
  Accession # Q07885
• Specificity: Detects feline IL‑2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross‑reactivity with recombinant canine IL-2 is observed, approximately 1% cross-reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed, and less than 0.2% cross‑reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Feline IL-2 Antibody (Catalog # AF1890)
  ELISA Detection: Feline IL-2 Biotinylated Antibody (Catalog # BAF1890)
  Standard: Recombinant Feline IL-2 (Cys146Ser) (Catalog # 1890-FL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody [Biotin]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4⁺ and CD8⁺ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha , beta , gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha , beta , gamma  subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta  and gamma  subunits. The non-ligand binding   gamma  subunit, referred to as the common gamma  subunit ( gamma _c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8⁺ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8⁺ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4⁺ CD25⁺ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1‑9).

1. Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
2. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
3. Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
5. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
7. Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
8. Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • Q07885()