IL-2 Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Rt
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 647

IL-2 Antibody [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects rat IL-2 in ELISAs. Detects human and rat IL-2 in Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse IL‑2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody [Alexa Fluor™ Plus 647]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature rat IL-2 shares 66% and 73% amino acid sequence identity with human and mouse IL-2, respectively. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (4-6). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta , which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain  gamma c/IL-2 R gamma , which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4⁺ T cells but not activated CD4⁺ memory lymphocytes (7). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (8-10). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (11, 12).

1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
3. McKnight, A. et al. (1989) Immunogenetics 30:145.
4. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
5. Wang, X. et al. (2005) Science 310:1159.
6. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
7. Jaleco, S. et al. (2003) J. Immunol. 171:61.
8. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
9. Laurence, A. et al. (2007) Immunity 26:371.
10. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
11. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
12. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.