IL-2 Antibody (JES6-1A12) [Unconjugated]

Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: JES6-1A12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-2 Antibody (JES6-1A12) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-2
• Specificity: Detects mouse IL-2 in ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.125-0.75 ug/mL
• Application Notes: ELISA Detection: Mouse IL-2 Biotinylated Antibody (Catalog number BAF402)
  Standard: Recombinant Mouse IL-2 (Catalog number 402-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody (JES6-1A12) [Unconjugated]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha , beta , and gamma  chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-2 Antibody (MAB702)(17)

Publications using MAB702

• Chun-Chi Chou, Kuo-Tai Hua, Min-Wei Chen, Chin-Jui Wu, Chun-Hua Hsu, Jann-Tay Wang et al. Discovery and characterization of a monoclonal antibody targeting a conformational epitope of IL-6/IL-6R alpha to inhibit IL-6/ IL-6R alpha /gp130 hexameric signaling complex formation mAbs 2022-12-31 [PMID: 35133941] (Neutralization, Mouse)
• Chun-Chi Chou, Kuo-Tai Hua, Min-Wei Chen, Chin-Jui Wu, Chun-Hua Hsu, Jann-Tay Wang et al. Discovery and characterization of a monoclonal antibody targeting a conformational epitope of IL-6/IL-6R alpha to inhibit IL-6/ IL-6R alpha /gp130 hexameric signaling complex formation mAbs 12/31/2022 [PMID: 35133941]
• W Piao, Y Xiong, L Li, V Saxena, KD Smith, KL Hippen, C Paluskievi, M Willsonshi, BR Blazar, R Abdi, JS Bromberg Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration Cell Rep, 2020-01-28;30(4):1052-1062.e5. 1/28/2020 [PMID: 31995749]
• B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC Oncogene, 2019-02-14;0(0):. 2019-02-14 [PMID: 30765860] (Neutralization, Mouse)
• Wei Cao, Hisako Kayama, Mei Lan Chen, Amber Delmas, Amy Sun, Sang Yong Kim et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids Immunity 2017-12-01 [PMID: 29262351]
• Amaia Zabala-Letona, Amaia Arruabarrena-Aristorena, Natalia Martín-Martín, Sonia Fernandez-Ruiz, James D. Sutherland, Michelle Clasquin et al. mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer Nature 2017-07-06 [PMID: 28658205]
• I Hwang, K Kim, S Choi, M Lomunova Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex Mol. Cells, 2017-01-31;40(1):24-36. 1/31/2017 [PMID: 28152301]
• Ivan D. Mascanfroni, Maisa C. Takenaka, Ada Yeste, Bonny Patel, Yan Wu, Jessica E. Kenison et al. Metabolic control of type 1 regulatory (Tr1) cell differentiation by AHR and HIF1-alpha Nature Medicine 2015-06-01 [PMID: 26005855]
• Simonetta F, Gestermann N, Bloquet S, Bourgeois C Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression. PLoS ONE, 2014-12-08;9(12):e113314. 2014-12-08 [PMID: 25485946] (Adoptive Transfer, Mouse)
• Francisco J Quintana, Hulin Jin, Evan J Burns, Meghan Nadeau, Ada Yeste, Deepak Kumar et al. Aiolos promotes TH17 differentiation by directly silencing Il2 expression Nature Immunology 2012-08-01 [PMID: 22751139]
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Ganesh BB, Bhattacharya P, Gopisetty A, Sheng J, Vasu C, Prabhakar BS IL-1beta promotes TGF-beta1 and IL-2 dependent Foxp3 expression in regulatory T cells. PLoS ONE, 2011-07-11;6(7):e21949. 7/11/2011 [PMID: 21779356]
• Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J., 2010-09-08;25(1):409-19. 2010-09-08 [PMID: 20826539] (Neutralization, Mouse)
• Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells. J. Immunol., 2010-01-18;184(4):2130-9. 2010-01-18 [PMID: 20083667] (Neutralization, Mouse)
• Ito H, Ando K, Ishikawa T Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance. Int. Immunol., 2008-05-16;20(7):869-79. 2008-05-16 [PMID: 18487227] (Neutralization, Mouse)
• Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse)
• Bednarski JJ, Warner RE, Rao T Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine. Arthritis Rheum., 2003-03-01;48(3):757-66. 2003-03-01 [PMID: 12632430] (ELISpot Development, Mouse)

Bioinformatics

• Gene Symbol: IL2