IL-2 Antibody (142206)

• Reactivity: Mu
• Applications: WB
• Clone: 142206
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-2 Antibody (142206) Summary

• Immunogen: E. coli-derived recombinant mouse IL-2
  Ala21-Gln169
  Accession # P04351
• Specificity: Detects mouse IL-2 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) IL‑2, rrIL‑2, rpIL‑2, rcrIL‑2, or rhIL‑15.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody (142206)

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha , beta , and gamma chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-2 Antibody (MAB402)(4)

Publications using MAB402

• I Hwang, K Kim, S Choi, M Lomunova Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex Mol. Cells, 2017-01-31;40(1):24-36. 2017-01-31 [PMID: 28152301] (ELISA Development (Capture), Mouse)
• Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. J. Immunol., 2007-02-15;178(4):2018-27. 2007-02-15 [PMID: 17277105] (Neutralization, Mouse)
• Zhou JY, Wang JY, Chen JG, Wu JX, Gong H, Teng QY, Guo JQ, Shen HG Cloning, in vitro expression and bioactivity of duck interleukin-2. Mol. Immunol., 2005-03-01;42(5):589-98. 2005-03-01 [PMID: 15607818] (Western Blot, Duck)
• Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem. J., 2004-06-01;380(0):425-33. 2004-06-01 [PMID: 14992689] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P04351()