IL-2 Antibody (100312)

• Reactivity: Po
• Applications: WB, CyTOF-ready, Flow
• Clone: 100312
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-2 Antibody (100312) Summary

• Immunogen: E. coli-derived recombinant porcine IL-2
  Ala21-Thr154
  Accession # P26891
• Specificity: Detects porcine IL-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human, mouse, rat, bovine, canine, equine, feline, or cotton rat IL-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody (100312)

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mature porcine and human IL-2 share approximately 72% amino acid sequence identity.The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex is also a subunit of IL-4, IL-7, and IL-9 receptor complexes.

1. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
2. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
3. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-2 Antibody (MAB6521)(1)

Publication using MAB6521

• Hernandez J, Soto-Canevett E, Pinelli-Saavedra A, Resendiz M, Moya-Camarena SY, Klasing KC In vitro effect of vitamin E on lectin-stimulated porcine peripheral blood mononuclear cells. Vet. Immunol. Immunopathol., 2009;131(1):9-16. 2009 [PMID: 19346007] (Flow Cytometry, Porcine)

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P26891()