IL-18/IL-1F4 Antibody (93-10C) [Biotin]

• Applications: ELISA(Det)
• Clone: 93-10C
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin

IL-18/IL-1F4 Antibody (93-10C) [Biotin] Summary

• Immunogen: Mouse IL-18/IL-1F4
• Specificity: This antibody reacts with mouse IL-18 on ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: This antibody solution is stable for one year from the date of purchase when stored at 4°C.
• Buffer: In 50 μL volume of PBS containing 1% BSA, 10% glycerol and 0.09% sodium azide, pH 7.2.
• Preservative: 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma . Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
7. Okamura, H., et al. Nature 378, 88-91 (1995)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL18