IL-18/IL-1F4 Antibody (314811) [Unconjugated]

IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB29241) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Ca
• Applications: WB, ICC/IF
• Clone: 314811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-18/IL-1F4 Antibody (314811) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant canine IL‑18/IL‑1F4
  Tyr37-Ser193
  Accession # Q9XSR0
• Specificity: Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (314811) [Unconjugated]

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18, also known as interferon-gamma-inducing factor (IGIF) or IL-1 gamma , is an 18 kDa member of the IL-1 superfamily which shares biologic activities with
IL‑12. IL-18 is expressed by liver cells, monocyte/macrophages, osteoblasts and keratinocytes. Mature canine IL-18 shares 73% sequence identity with human IL-18. Caspase-1 (IL-1 beta-converting enzyme) has been shown to process pro-IL-18 to active IL-18. Human IL-18 has been shown to enhance NK cell activity, induce the production of IFN-gamma and GM-CSF and inhibit the production of IL-10 by PBMCs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL18
• Uniprot:
  • Q9XSR0()