IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, B/N
• Clone: 125-2H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free (NBP2-81040) is a recombinant monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion of splenocytes from mice immunized with human IL-18/IL-1F4, with SP2/O mouse myeloma cells.
• Specificity: Recognizes the active form of human IL-18/IL-1F4.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL18
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Neutralization
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

The protein encoded by the IL18 gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL18